The germinal center response is impaired in the absence of T cell-expressed CXCR5
The germinal center response is impaired in the absence of T cell-expressed CXCR5
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DOI：
10.1002/eji.200636486
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发表时间：
2007-01-01
影响因子：
5.4
通讯作者：
Butcher, Eugene C.
中科院分区：
文献类型：
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作者：
Arnold, Carrie N.;Campbell, Daniel J.;Butcher, Eugene C.
在感染期间或接种疫苗后，衰老中心支持记忆B细胞和长寿抗体分泌细胞的分化。在这里，我们用选择性缺乏趋化因子受体CXCR5的T细胞构建小鼠，以确定T细胞表达这种受体是否是生发中心形成和功能的必需条件。在这些动物中，用胸腺依赖性抗原免疫后，正确定位于B细胞滤泡中并含有T细胞的生发中心确实形成。然而，更少和更小的生发中心形成，导致生发中心B细胞的频率显著降低。脾脏和骨髓中同种型转换的抗体分泌细胞频率降低以及血清中总IgG和高亲和力半抗原特异性IgG浓度降低可弥补生发中心形成缺陷（1）。结果表明，虽然CXCR5依赖性T细胞定位是重要的最大诱导和扩增的生发中心，刺激同种型类转换，并开发抗体分泌细胞的种子脾脏和骨髓，它不是绝对需要的滤泡生发中心的形成和功能。
Germinal centers support the differentiation of memory B cells and long-lived antibody-secreting cells during infection or upon vaccination. Here, we constructed mice with T cells that selectively lack the chemokine receptor CXCR5 to determine if expression of this receptor by T cells is mandatory for germinal center formation and function. In these animals, germinal centers that are properly localized in B cell follicles and contain T cells do form after immunization with a thymus-dependent antigen. However, fewer and smaller germinal centers form, resulting in a significant reduction in the frequency of germinal center B cells. The defect in germinal center formation is paralleled by decreased frequencies of isotype-switched antibody-secreting cells in the spleen and bone marrow and reduced serum concentrations of total and high-affinity hapten-specific IgG(1). The results demonstrate that although CXCR5-dependent T cell positioning is important for maximal induction and expansion of germinal centers, stimulation of isotype class switching, and development of antibody-secreting cells that seed the spleen and bone marrow, it is not absolutely required for the formation and function of follicular germinal centers.