ACETYLATED ALPHA-TUBULIN IN MICROTUBULES DURING MOUSE FERTILIZATION AND EARLY DEVELOPMENT
ACETYLATED ALPHA-TUBULIN IN MICROTUBULES DURING MOUSE FERTILIZATION AND EARLY DEVELOPMENT
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DOI：
10.1016/0012-1606(88)90415-0
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发表时间：
1988-11-01
影响因子：
2.7
通讯作者：
SCHATTEN, H
中科院分区：
文献类型：
--
作者：
SCHATTEN, G;SIMERLY, C;SCHATTEN, H
.alpha。小鼠卵母细胞和胚胎微管中的微管蛋白在特定的空间和时间序列中乙酰化。在未受精的卵母细胞中，一种针对。α乙酰化形式的单克隆抗体。-微管蛋白主要结合在中期减数分裂纺锤体的两极。用双标记总微管蛋白和乙酰化α的卵母细胞进行免疫荧光观察，纺锤体微管的标记强度较弱。-微管蛋白，用胶体金免疫高压电子显微镜（immunoHVEM）测定；cytaster没有乙酰化。在减数分裂后期，纺锤体被标记，在减数分裂末期和第二极体形成期间，只有减数分裂中体被乙酰化。精子轴素在掺入后仍保持乙酰化，但间期微管未被检测到。第一次有丝分裂遵循与第二次减数分裂相似的模式，在间期只有有丝分裂的中间体被乙酰化。在用冷、秋碱或灰黄霉素处理后，剩余的稳定微管被乙酰化，但免疫hvem观察表明，这些纤维在微管破坏之前可能没有乙酰化。紫杉醇稳定不改变乙酰化模式。乙酰化微管不一定是老微管，因为在冷恢复后30秒观察到乙酰化纤维。这些结果表明，在减数分裂和有丝分裂期间存在乙酰化的微管，并证明了细胞周期特异性的乙酰化模式，在中期中心体中发现乙酰化的微管，在后期纺锤体标记增加，在末期除了中间体外的所有微管都选择性地去乙酰化。
.alpha.-Tubulin in the microtubules of mouse oocytes and embryos is acetylated in a specific spatial and temporal sequence. In the unfertilized oocyte, a monoclonal antibody to the acetylated form of .alpha.-tubulin is bound predominantly at the poles of the arrested metaphase meiotic spindle. The labeling intensity of the spindle microtubules is weaker as observed by immunofluorescence using oocytes double-labeled for total tubulin and acetylated .alpha.-tubulin, and as measured by immuno high-voltage electron microscopy (immunoHVEM) with colloidal gold; cytasters are not acetylated. At meiotic anaphase, teh spindle becomes labeled, and by telophase and during second polar body formation only the meiotic midbody is acetylated. The sperm axoneme retains its acetylation after incorporation though the interphase microtubules are not detected. First mitosis follows a pattern similar to that observed at the second meiosis and during interphase only the mitotic midbodies are acetylated. After treatment with cold, colcemid, or griseofulvin, the remaining stable microtubules are acetylated, but immunoHVEM observations suggest that these fibers might not have been acetylated prior to microtubule disruption. Taxol stabilization does not alter acetylation pattern. Acetylated microtubules are not necessarily old microtubules since acetylated fibers are observed at 30 sec after cold recovery. These results show the presence of acetylated microtubules during meiosis and mitosis and demonstrate a cell-cycle-specific pattern of acetylation, which acetylated microtubules found at the centrosomes at metaphase, an increase in spindle labeling at anaphase and the selective deacetylation of all but midbody microtubules at telophase.