Copper is required for prion protein‐associated superoxide dismutase‐l activity in Pichia pastoris
Copper is required for prion protein‐associated superoxide dismutase‐l activity in Pichia pastoris
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毕赤酵母中朊病毒蛋白相关的超氧化物歧化酶-l活性需要铜
DOI：
10.1111/j.1742-4658.2007.05678.x
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
G. Multhaup
中科院分区：
文献类型：
--
作者：
C. Treiber;R. Pipkorn;C. Weise;Gudrun Holland;G. Multhaup
朊病毒蛋白（PrP）是与传染性海绵状脑病有关的关键蛋白。它是一种结合锰和铜的金属蛋白。后者参与蛋白质的生理功能。我们之前已经发现，巴斯德毕赤酵母中的PrP表达影响细胞内金属离子浓度，并且蛋白酶抗性PrP的形成由额外的铜和/或锰诱导。在这项研究中，我们表明异源表达的PrP是后修饰和运输到细胞壁。我们通过结合三种不同的测试系统发现，PrP本身在巴斯德毕赤酵母中获得了超氧化物歧化酶样活性。 然而，该活性不能被KCN抑制，并且依赖于培养基中额外的铜。因此，本研究通过显示铜必须存在于蛋白质中以参与活性氧的清除和解毒来定义PrP表现出超氧化物歧化酶样活性的条件。
The prion protein (PrP) is the key protein implicated in transmissible spongiform encephalopathies. It is a metalloprotein that binds manganese and copper. The latter is involved in the physiological function of the protein. We have previously found that PrP expression in Pichia pastoris affects intracellular metal ion concentrations and that formation of protease‐resistant PrP is induced by additional copper and/or manganese. In this study, we show that heterologously expressed PrP is post‐translationally modified and transported to the cell wall. We found by combining three different test systems that PrP itself had gained superoxide dismutase‐like activity in P. pastoris. However, this activity could not be inhibited by KCN and depended on additional copper in the medium. Thus, this study defines the conditions under which PrP exhibits superoxide dismutase‐like activity by showing that copper must be present for the protein to participate in scavenging and detoxification of reactive oxygen species.
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影响因子：
--
作者：
S. Prusiner
通讯作者：
S. Prusiner
DOI：
10.1073/pnas.83.8.2310
发表时间：
1986-04-01
影响因子：
11.1
作者：
MEYER, RK;MCKINLEY, MP;PRUSINER, SB
通讯作者：
PRUSINER, SB
影响因子：
3.3
作者：
Stewart, RS;Drisaldi, B;Harris, DA
通讯作者：
Harris, DA
影响因子：
2.9
作者：
Stöckel, J;Safar, J;Prusiner, SB
通讯作者：
Prusiner, SB
影响因子：
2.9
作者：
STAHL, N;BALDWIN, MA;PRUSINER, SB
通讯作者：
PRUSINER, SB