Changes in neuronal migration in neocortex of connexin43 null mutant mice
Changes in neuronal migration in neocortex of connexin43 null mutant mice
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DOI：
10.1093/jnen/62.3.304
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发表时间：
2003-03-01
影响因子：
3.2
通讯作者：
Naus, CCG
中科院分区：
文献类型：
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作者：
Fushiki, S;Velazquez, JLP;Naus, CCG
为了鉴定连接蛋白43无效突变小鼠的神经表型，在发育中的新皮层中研究了电生理特性、细胞间通讯和神经元迁移。在新生小鼠的急性切片制备中，野生型和无效突变小鼠之间的皮层和海马神经元的电生理特性没有显着差异。然而，间隙连接耦合评估光漂白后的荧光恢复显着衰减无效突变小鼠的新皮层脑切片。为了评估神经元迁移，分裂细胞标记溴脱氧尿苷（BrdU）在胚胎第12天，14日和16日，分别对应于皮质神经发生的时期，和新皮质检查后2或3天的标签。BrdU标记的细胞分布在新皮质壁与一个显着的变化，在新皮质的无效突变体，标记的细胞积累在中间区或在皮质板的内部的图案。结果表明，在新皮层神经元迁移的connexin43无效突变体的显着延迟，和connexin43在这一过程中可能发挥的作用，通过尚未确定的机制。
To identify a neural phenotype in connexin43 null mutant mice, electrophysiological properties, intercellular communication and neuronal migration were studied in the developing neocortex. In acute slice preparations from newborn mice, electrophysiological characteristics of cortical and hippocampal neurons were not significantly different between wild type and null mutant mice. However, gap junctional coupling as assessed by fluorescence recovery after photobleaching was significantly attenuated in neocortical brain slices of null mutant mice. To assess neuronal migration, dividing cells were labeled with bromodeoxyuridine (BrdU) on embryonic days 12, 14 and 16, respectively, corresponding to the period of cortical neurogenesis, and the neocortex examined 2 or 3 days after the labeling. BrdU-labeled cells were distributed in the neocortical wall with a significant change in the pattern in the neocortex of the null mutant, where labeled cells accumulated in the intermediate zone or in the inner part of the cortical plate. The result suggests a significant delay in neocortical neuronal migration in the connexin43 null mutants, and a possible role of connexin43 in this process through yet unidentified mechanisms.