Preparation of gold nanocluster bioconjugates for electron microscopy.
Preparation of gold nanocluster bioconjugates for electron microscopy.
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DOI：
10.1007/978-1-62703-137-0_17
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发表时间：
2013-01-01
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影响因子：
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通讯作者：
Ackerson, Christopher J
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文献类型：
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作者：
Heinecke, Christine L;Ackerson, Christopher J
在本章中，我们描述金纳米粒子-生物分子缀合物的类型及其在电子显微镜中的用途。其中包括两个用单层金保护簇标记 IgG 抗体的详细方案。第一种方法是利用配体位置交换反应的直接键合方法。第二种方法描述了 Au(144)(pMBA)(60) 与 IgG 的 NHS-EDC 偶联。还包括用于确定标记效率的各种表征技术。
In this chapter, we describe types of gold nanoparticle-biomolecule conjugates and their use in electron microscopy. Included are two detailed protocols for labeling an IgG antibody with gold monolayer protected clusters. The first approach is a direct bonding approach that utilizes the ligand place exchange reaction. The second approach describes NHS-EDC coupling of Au(144)(pMBA)(60) with IgG. Also included are various characterization techniques for determining labeling efficiency.