TCP transcription factor, BRANCH ANGLE DEFECTIVE 1 (BAD1), is required for normal tassel branch angle formation in maize
TCP transcription factor, BRANCH ANGLE DEFECTIVE 1 (BAD1), is required for normal tassel branch angle formation in maize
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DOI：
10.1073/pnas.1202439109
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发表时间：
2012-07-24
影响因子：
11.1
通讯作者：
Schmidt, Robert J.
中科院分区：
文献类型：
--
作者：
Bai, Fang;Reinheimer, Renata;Schmidt, Robert J.
在草的花序中，在花序主茎和侧枝之间发现了一种称为“枕”的结构。叶枕的大小影响从主轴出现的侧枝的角度，因此对花序结构有很大的影响。通过EMS诱变，我们鉴定了三组具有叶枕形成缺陷的玉米隐性突变体。所有的突变体都表现出非常尖锐的雄穗分支角伴随着一个显着的减少，与正常植物相比，在枕的大小。其中两个互补组对应于先前鉴定的基因RAMOSA 2（RA 2）和LIGULELESS 1（LG 1）中的突变。使用基于图谱的方法克隆了对应于第三组的突变体，并发现其编码DNA结合蛋白的植物特异性TCP（TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL NUCLEAR ANGEEN FACTOR）家族的新成员，分支角缺陷1（BAD 1）。BAD 1在发育中的叶枕以及其他发育中的组织中表达，包括雄穗和幼叶。分子和遗传学研究表明，RA 2是上游的BAD 1，而LG 1可能在一个单独的途径发挥作用。我们的研究结果表明，BAD 1是一个TCP II类基因，其功能是促进细胞增殖的侧器官，枕，并影响花序结构的影响的角度，侧分支出现。
In grass inflorescences, a structure called the "pulvinus" is found between the inflorescence main stem and lateral branches. The size of the pulvinus affects the angle of the lateral branches that emerge from the main axis and therefore has a large impact on inflorescence architecture. Through EMS mutagenesis we have identified three complementation groups of recessive mutants in maize having defects in pulvinus formation. All mutants showed extremely acute tassel branch angles accompanied by a significant reduction in the size of the pulvinus compared with normal plants. Two of the complementation groups correspond to mutations in the previously identified genes, RAMOSA2 (RA2) and LIGULELESS1 (LG1). Mutants corresponding to a third group were cloned using mapped-based approaches and found to encode a new member of the plant-specific TCP (TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL NUCLEAR ANTIGEN FACTOR) family of DNA-binding proteins, BRANCH ANGLE DEFECTIVE 1 (BAD1). BAD1 is expressed in the developing pulvinus as well as in other developing tissues, including the tassels and juvenile leaves. Both molecular and genetics studies show that RA2 is upstream of BAD1, whereas LG1 may function in a separate pathway. Our findings demonstrate that BAD1 is a TCP class II gene that functions to promote cell proliferation in a lateral organ, the pulvinus, and influences inflorescence architecture by impacting the angle of lateral branch emergence.