Anticholinesterase and mutagenic evaluation of in vitro-regenerated Agapanthus praecox grown ex vitro
Anticholinesterase and mutagenic evaluation of in vitro-regenerated Agapanthus praecox grown ex vitro
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DOI：
10.1007/s11627-013-9567-z
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发表时间：
2014-03-01
影响因子：
2.6
通讯作者：
Van Staden, Johannes
中科院分区：
文献类型：
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作者：
Baskaran, Ponnusamy;Chukwujekwu, Jude C.;Van Staden, Johannes
除了建立药用植物的微繁殖方案外，重要的是要确定繁殖植物的功效和安全性，以便这些植物被接受用于传统医学。使用繁殖植物可以减轻/减少对野生种群的过度开发。本研究评价了1年生百子莲离体培养和自然生长母株的抗胆碱酯酶和致突变特性。使用不同的植物生长调节剂再生组织培养衍生的植物。乙酰胆碱酯酶（AChE）酶的剂量依赖性抑制中观察到的所有组织培养衍生的和自然生长的母株。苄基腺嘌呤（BA）和噻苯隆（TDZ）的组合再生的组织培养衍生的植物的叶提取物表现出显着低的AChE抑制活性。相反，与其他处理和自然生长的母株的提取物相比，单独使用BA再生的植物根提取物表现出最高的AChE抑制活性（IC 50 = 0.20 mg/mL）。在不存在代谢活化的情况下，未发现任何样品具有致突变性。本研究表明，再生植物可作为传统医学中天然植物的有效替代品。然而，在微繁殖操作过程中使用的治疗的选择可能会显着影响再生植物的治疗潜力，即使在1年的增长。
Beyond establishing micropropagation protocols for medicinal plants, it is important that the efficacy and safety of propagated plants be ascertained for these plants to be accepted for use in traditional medicine. The use of propagated plants could alleviate/reduce over-exploitation of wild populations. The present study evaluated the anticholinesterase and mutagenic properties of 1-yr-old tissue culture-derived Agapanthus praecox grown ex vitro and naturally grown mother plants. The tissue culture-derived plants were regenerated using different plant growth regulators. A dose-dependent inhibition of acetylcholinesterase (AChE) enzyme was observed in all the tissue culture-derived and naturally grown mother plants. The leaf extract of tissue culture-derived plants regenerated with a combination of benzyladenine (BA) and thidiazuron (TDZ) demonstrated a significantly low AChE-inhibitory activity. Conversely, the root extract of plants regenerated with BA alone demonstrated the highest AChE-inhibition activity (IC50 = 0.20 mg/mL) when compared to extracts from other treatments and the naturally grown mother plants. None of the samples were found to be mutagenic in the absence of metabolic activation. The present study indicated that regenerated plants could be used as potent substitutes for naturally grown plants in traditional medicine. However, the choice of treatment used during micropropagation operation may significantly influence the therapeutic potential of regenerated plants, even after 1 yr of growth.