Maintenance of size and function of influenza virus hemagglutinin specific transgenic T-cell clone during life.
Maintenance of size and function of influenza virus hemagglutinin specific transgenic T-cell clone during life.
复制标题
流感病毒血凝素特异性转基因 T 细胞克隆在生命过程中维持大小和功能。
DOI：
10.1111/j.1582-4934.2001.tb00173.x
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发表时间：
2001
影响因子：
5.3
通讯作者：
Bona,CA
中科院分区：
文献类型：
--
作者：
Radu,DL;Weksler,ME;Bona,CA
与年轻受试者相比，老年人对传染病的保护性免疫能力较弱。我们研究了年龄对表达流感血凝素110-120肽受体的转基因小鼠T细胞体外和体内功能的影响。在衰老过程中，转基因T细胞经历了与年龄相关的从幼稚表型到记忆表型的转变，但在体外，尽管胸腺退化，它们的数量以及血凝素110-120肽诱导的细胞因子产生和增殖反应仍保持不变。在衰老过程中，转基因T细胞的大小和功能的维持可能与CTLA-4分子的低表达有关，CTLA-4分子在与共刺激分子相互作用后表现出负调节作用，以及未知的交叉反应内源性因素刺激T细胞，而不是名义抗原，因为先天免疫阻止了小鼠流感病毒的自然感染。提示老年受试者免疫低下反映的是T细胞记忆发育和维持的缺陷，而不是效应器活性的表达缺陷。
Immunization induces less protective immunity against infectious diseases in old compared to young subjects. We have studied the effect of age on thein vitroandin vivofunction of murine transgenic T cells expressing a receptor for influenza hemagglutinin 110‐120 peptide. During aging the transgenic T cells undergo the age‐associated shift from naive to memory phenotype but maintain, despite thymic involution, their number as well as their cytokine production and proliferative responses induced by the hemagglutinin 110‐120 peptidein vitro. The maintenance of the size and functions of transgenic T cells during the aging may be related to low expression of CTLA‐4 molecules known to exhibit a negative regulatory effect subsequent to interaction with costimulatory molecules as well as of stimulation of T cells by unknown cross reactive endogenous factors but not by nominal antigen since innate immunity prevents natural infection with influenza virus of murine species. This suggests that the impaired immunity induced by immunization in old subjects reflects defects in the development and maintenance of T cell memory and not in the expression of effector activity.