Mitochondria Regulate the Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth
Mitochondria Regulate the Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth
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DOI：
10.1247/csf.17012
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发表时间：
2017-01-01
影响因子：
1.5
通讯作者：
Nonaka, Kazuaki
中科院分区：
文献类型：
--
作者：
Kato, Hiroki;Thanh Thi Mai Pham;Nonaka, Kazuaki
人脱落乳牙干细胞（SHED）是从乳牙牙髓组织中分离出来的，可以分化为神经细胞。尽管SHED是用于移植治疗和用于神经系统疾病研究的理想类型的干细胞，但SHED的神经元分化机制的大部分仍不清楚。最近的研究表明，线粒体活性参与干细胞的分化。在目前的工作中，我们调查的神经元分化机制的SHED集中在线粒体活性。在SHED的神经元分化过程中，我们观察到线粒体膜电位增加，线粒体DNA增加，线粒体延长。此外，为了研究神经元分化中线粒体活性的需求，我们在鱼藤酮（线粒体呼吸链复合物I的抑制剂）和羰基氰间氯苯腙（CCCP）（线粒体解偶联剂）的存在下将SHED分化为神经元细胞，并发现用鱼藤酮和CCCP处理抑制了神经元分化。这些结果表明，线粒体活性的增加是至关重要的神经元分化的SHED。
Stem cells from human exfoliated deciduous teeth (SHED) are isolated from the dental pulp tissue of primary teeth and can differentiate into neuronal cells. Although SHED are a desirable type of stem cells for transplantation therapy and for the study of neurological diseases, a large part of the neuronal differentiation machinery of SHED remains unclear. Recent studies have suggested that mitochondrial activity is involved in the differentiation of stem cells. In the present work, we investigated the neuronal differentiation machinery of SHED by focusing on mitochondrial activity. During neuronal differentiation of SHED, we observed increased mitochondrial membrane potential, increased mitochondrial DNA, and elongated mitochondria. Furthermore, to examine the demand for mitochondrial activity in neuronal differentiation, we then differentiated SHED into neuronal cells in the presence of rotenone, an inhibitor of mitochondrial respiratory chain complex I, and carbonyl cyanide m-chlorophenyl hydrazone (CCCP), a mitochondrial uncoupler, and found that neuronal differentiation was inhibited by treatment with rotenone and CCCP. These results indicated that increased mitochondrial activity was crucial for the neuronal differentiation of SHED.