Whole-mount in situ hybridization and detection of RNAs in vertebrate embryos and isolated organs.
Whole-mount in situ hybridization and detection of RNAs in vertebrate embryos and isolated organs.
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DOI：
10.1002/0471142727.mb1409s66
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发表时间：
2004-05-01
影响因子：
--
通讯作者：
Paganelli, Alejandra
中科院分区：
文献类型：
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作者：
Pizard, Anne;Haramis, Anna;Paganelli, Alejandra
利用完整胚胎或器官进行非同位素原位杂交是确定rna空间分布的重要方法。因为它允许分析大量样本，它适用于时间表达研究和不同基因型之间的比较。它提供了灵敏度和再现性。此外，在染色过程中组织学细节不会丢失。本单元的方案可用于爪蟾、小鼠和鸡胚胎的全贴装原位杂交，也可用于小鼠和鸡的解剖器官。还描述了地高辛标记的核糖探针的制备。
Nonisotopic in situ hybridization using intact embryos or organs is an important method for determining the spatial distribution of RNAs. Because it allows the analysis of large numbers of samples, it is amenable to temporal expression studies and comparison between different genotypes. It offers sensitivity and reproducibility. In addition, histological details are not lost during the staining process. The protocols in this unit can be used for whole-mount in situ hybridization in Xenopus, mouse, and chicken embryos, as well as dissected organs from mouse and chicken. Preparation of digoxigenin-labeled riboprobes is also described.