Microbicidal action of photoirradiated aqueous extracts from wine lees
Microbicidal action of photoirradiated aqueous extracts from wine lees
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DOI：
10.1007/s13197-016-2273-1
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发表时间：
2016-07-01
影响因子：
3.1
通讯作者：
Niwano, Yoshimi
中科院分区：
文献类型：
--
作者：
Tsukada, Mana;Sheng, Hong;Niwano, Yoshimi
酒糟是酿酒过程中的主要废物，是多酚化合物的丰富来源。 400 nm 的 LED 光照射引发了酒糟水提取物 (WLE) 对金黄色葡萄球菌、铜绿假单胞菌和白色念珠菌的杀菌活性，此外还形成了活性氧 (ROS)，包括羟基自由基 ((OH)-O-中心点) 和过氧化氢 (H2O2)。尽管单独进行 20 分钟的光照射处理即可发挥杀菌活性，减少 2 至 3 个对数级，但光照射 20 分钟的 WLE 可使活的金黄色葡萄球菌和铜绿假单胞菌细胞减少 5 个对数级或更多。对于白色念珠菌，光照射 WLE 40 分钟可实现活细胞减少 1 个对数（减少 90%），而单独光照射并未显示出任何杀菌效果。 ROS 分析显示，光照射 WLE 20 分钟后产生约 170 μM 中心点 OH 和 600 μM H2O2。由于光照射WLE的杀菌活性被中心点OH清除剂消除，ROS，尤其是高氧化中心点OH，可能是光照射WLE的杀菌活性的原因。除了杀菌活性外，WLE 还可以充当抗氧化剂，因为它对 2,2-二苯基-1-三硝基苯肼（一种稳定的自由基）具有自由基清除活性。
Wine lees, a major waste product of winemaking, is a rich source of polyphenolic compounds. LED-light irradiation at 400-nm elicited microbicidal activity of aqueous extract from wine lees (WLE) against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans, in addition to reactive oxygen species (ROS) formation, including hydroxyl radical ((OH)-O-center dot) and hydrogen peroxide (H2O2). Although treatment for 20 min of photoirradiation alone exerted bactericidal activity with a 2- to 3-log reduction, photoirradiated WLE for 20 min achieved a 5-log or greater reduction in viable S. aureus and P. aeruginosa cells. Regarding C. albicans, a 1-log reduction (90 % reduction) of viable cells was achieved by photoirradiated WLE for 40 min, whereas photoirradiation alone did not show any fungicidal effect. ROS analyses revealed that approximately 170 mu M center dot OH and 600 mu M H2O2 were generated in photoirradiated WLE for 20 min. Because the bactericidal activity of photoirradiated WLE was abolished by center dot OH scavengers, ROS, especially highly oxidative center dot OH, may be responsible for the microbicidal activity of photoirradiated WLE. In addition to its microbicidal activity, WLE may act as an antioxidant as it exerted radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl, a stable free radical.