Upregulation of KIF20A correlates with poor prognosis in gastric cancer
Upregulation of KIF20A correlates with poor prognosis in gastric cancer
复制标题
KIF20A 上调与胃癌不良预后相关
DOI：
10.2147/cmar.s176147
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发表时间：
2018-01-01
影响因子：
3.3
通讯作者：
Li, Yongxiang
中科院分区：
文献类型：
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作者：
Sheng, Yi;Wang, Wei;Li, Yongxiang
背景KIF20A被认为是有丝分裂的关键蛋白之一。最近，许多研究表明，KIF20A可能在一些癌症中起癌基因的作用。然而，其在胃癌中的表达水平和临床价值尚不清楚。患者和方法在本研究中，我们用实时定量聚合酶链式反应和免疫印迹方法检测了KIF20A在胃癌患者和细胞系中的表达。通过细胞存活率和集落形成实验检测KIF20A在胃癌细胞增殖中的作用。应用免疫组织化学方法检测146例KIF20A的预后价值。同时对122例基于KIF20A表达的胃癌患者的总体生存率进行分析。最后，用KIF20A抑制剂金雀异黄素与顺铂或氟尿嘧啶联合应用，研究其抗肿瘤作用。结果大多数(56.76%)胃癌组织中KIF20A的表达水平高于相应的正常组织，提示KIF20A在胃癌中具有潜在的致癌作用。功能研究阐明了KIF20A在胃癌细胞增殖中的重要作用。此外，组织芯片结果显示KIF20A的表达水平与组织学分级显著相关(P=0.036)。此外，我们还发现KIF20A的表达与总的生存率有关，这与Kaplan-Meier绘图仪数据库的结果是一致的。此外，我们还发现KIF20A抑制剂金雀异黄素可以增强顺铂和氟尿嘧啶的抗肿瘤活性，这可能是一种GC化疗增敏药物。结论KIF20A可促进胃癌细胞的增殖，可作为一种独立的预后因素和潜在的治疗靶点。
Background KIF20A is well known as one of the key proteins in mitosis. Recently, a number of studies illustrated that KIF20A might function as an oncogene in some carcinomas. However, its expression levels and clinical value remained unclear in gastric cancer (GC). Patients and methods In this study, we investigated the expression of KIF20A in samples from GC patients and cell lines by quantitative real-time PCR and Western blot. The function of KIF20A in cell proliferation of GC cell lines was examined via cell viability and colony formation assays. Immunohistochemistry assay based on a tissue microarray consisting of 146 cases was performed to evaluate the prognostic value of KIF20A. The overall survival rate of 122 GC patients based on KIF20A expression was analyzed as well. Finally, using KIF20A inhibitor, genistein, and combining it with cisplatin or fluorouracil, the antitumor effects were studied. Results Most GC samples (56.76%) showed higher KIF20A expression level compared to their corresponding normal specimens, which demonstrated the potential oncogenic role of KIF20A in GC. The functional studies elucidated the essential role of KIF20A in GC cell proliferation. Besides, tissue microarray result showed that the expression level of KIF20A was significantly related to the histological grades (P=0.036). Furthermore, we found the expression of KIF20A was related to poor overall survival rate, which is coincident with the results from Kaplan–Meier plotter database. In addition, we found that a KIF20A inhibitor, genistein, could enhance the antitumor activity of cisplatin and fluorouracil, which might be considered as a chemosensitive agent in GC. Conclusion KIF20A can promote cell proliferation in GC, which might be used as an independent prognostic factor and a potential therapeutic target.