Stability of mitochondrial membrane proteins in terrestrial vertebrates predicts aerobic capacity and longevity.
Stability of mitochondrial membrane proteins in terrestrial vertebrates predicts aerobic capacity and longevity.
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DOI：
10.1093/gbe/evr079
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发表时间：
2011
影响因子：
3.3
通讯作者：
Tanaka M
中科院分区：
文献类型：
--
作者：
Kitazoe Y;Kishino H;Hasegawa M;Matsui A;Lane N;Tanaka M
线粒体产生的细胞能量是生命的基本货币。然而，线粒体（mt）性能（功率和耐力）在多大程度上适应脊椎动物的栖息地和生活策略尚不清楚。对 mt 基因组的全球分析表明，陆生脊椎动物的 mt 膜蛋白 (MMP) 疏水性 (HYD) 远低于鱼类，并且与丝氨酸/苏氨酸组成 (STC) 呈强烈负相关。在这里，我们提出的证据表明，MMP 的这种系统特征对于具有高有氧能力的大型陆生脊椎动物的进化至关重要。阿累尼乌斯型方程给出了陆地脊椎动物的 STC 与最大寿命 (MLS) 之间的正相关性（除了少数与静息代谢率高的小动物的生活方式有关的例外 [RMR]），以及次生海洋脊椎动物（例如鲸目动物和短吻鳄（它们利用体型增大的浮力从陆地返回水中））的负相关性。特别是，灵长类动物（尤其是类人猿）胎盘中 STC 的显着增加与非常高的 MLS 值相关。我们通过将 mtRMR 加速到 mt 最大代谢率来估计阿伦尼乌斯图的退化，从而将 MMP 中的这些 STC 增加与呼吸复合物的更高稳定性联系起来。陆生脊椎动物的 mtRMR 和 HYD 均随着体重的增加而降低。当不需要高迁移率时，mtRMR 的减少会提高 MMP 的稳定性，而 HYD 的减少可能会削弱脂质双层疏水环境下的这种稳定性。我们假设较高的最大代谢率（5-10 RMR）需要较高的 MMP 迁移率，这可能会使 MMP 更加不稳定。因此，STC 的显着升高可能对于通过与丝氨酸/苏氨酸基序相关的氢键稳定 MMP（可能作为动态超级复合物）至关重要。
The cellular energy produced by mitochondria is a fundamental currency of life. However, the extent to which mitochondrial (mt) performance (power and endurance) is adapted to habitats and life strategies of vertebrates is not well understood. A global analysis of mt genomes revealed that hydrophobicity (HYD) of mt membrane proteins (MMPs) is much lower in terrestrial vertebrates than in fishes and shows a strong negative correlation with serine/threonine composition (STC). Here, we present evidence that this systematic feature of MMPs was crucial for the evolution of large terrestrial vertebrates with high aerobic capacity. An Arrhenius-type equation gave positive correlations between STC and maximum life span (MLS) in terrestrial vertebrates (with a few exceptions relating to the lifestyle of small animals with a high resting metabolic rate [RMR]) and negative correlations in secondary marine vertebrates, such as cetaceans and alligators (which returned from land to water, utilizing buoyancy with increased body size). In particular, marked STC increases in primates (especially hominoids) among placentals were associated with very high MLS values. We connected these STC increases in MMPs with greater stability of respiratory complexes by estimating the degradation of the Arrhenius plot given by accelerating mtRMR up to mt maximum metabolic rate. Both mtRMR and HYD in terrestrial vertebrates decreased with increasing body mass. Decreases in mtRMR raise MMP stability when high mobility is not required, whereas decreased HYD may weaken this stability under the hydrophobic environment of lipid bilayer. High maximal metabolic rates (5–10 RMR), which we postulate require high MMP mobility, presumably render MMPs more unstable. A marked rise in STC may therefore be essential to stabilize MMPs, perhaps as dynamic supercomplexes, via hydrogen bonds associated with serine/threonine motifs.
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影响因子：
5.6
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.97.11.5796
发表时间：
2000-05-23
影响因子：
11.1
作者：
Eilers, M;Shekar, SC;Fleming, PJ
通讯作者：
Fleming, PJ