Direct DNA Conjugation to Star Polymers for Controlled Reversible Assemblies
Direct DNA Conjugation to Star Polymers for Controlled Reversible Assemblies
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DOI：
10.1021/bc200240q
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发表时间：
2011-10-01
影响因子：
4.7
通讯作者：
Das, Subha R.
中科院分区：
文献类型：
--
作者：
Averick, Saadyah;Paredes, Eduardo;Das, Subha R.
聚合物生物分子杂化物代表了一类强大的高度可定制的纳米材料。在这里，我们报告了使用“无配体”Cu（I）促进的叠氮化物-炔环加成点击反应与DNA的星形聚合物缀合物。星型聚合物结构的多价性允许其它分子沿着与DNA缀合，并且缀合方法提供对DNA取向的控制。星型聚合物DNA纳米粒子显示组装成更高阶的纳米组装体通过杂交。此外，我们表明，DNA链可用于控制拆卸的纳米结构。
Polymer biomolecule hybrids represent a powerful class of highly customizable nanomaterials. Here, we report star-polymer conjugates with DNA using a "ligandless" Cu(I) promoted azide-alkyne cycloaddition click reaction. The multivalency of the star-polymer architecture allows for the concomitant conjugation of other molecules along with the DNA, and the conjugation method provides control over the DNA orientation. The star-polymer DNA nanoparticles are shown to assemble into higher-order nanoassemblies through hybridization. Further, we show that the DNA strands can be utilized in controlled disassembly of the nanostructures.