Basic fibroblast growth factor production in vitro by macrophages exposed to Dacron and polyglactin 910.
Basic fibroblast growth factor production in vitro by macrophages exposed to Dacron and polyglactin 910.
复制标题
暴露于涤纶和聚乳酸 910 的巨噬细胞在体外产生碱性成纤维细胞生长因子。
DOI：
10.1163/156856293x00096
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Lam,TM
中科院分区：
文献类型：
--
作者：
Greisler,HP;Henderson,SC;Lam,TM
巨噬细胞激活植入的生物接触材料调节平滑肌细胞和内皮细胞向内生长。本研究评价了涤纶或polyglactin 910与来自正常或致动脉粥样硬化饮食家兔的巨噬细胞之间的体外相互作用。在存在或不存在（阴性对照）任一生物材料的情况下培养腹膜巨噬细胞7周。使用兔主动脉平滑肌细胞生物测定法（有或无中和抗碱性FGF抗体预孵育）评价条件培养基的促有丝分裂活性。结果表明，从动脉粥样硬化兔收获的巨噬细胞中释放的丝裂原增加。与对照组相比，暴露于聚乳糖910（p < 0.05）或涤纶（p <0.005）后，仅从正常饮食喂养的家兔中采集的巨噬细胞增加了其促分裂原释放。polyglactin 910对有丝分裂原释放的刺激作用并未显著超过涤纶。在喂食正常饮食的家兔中，用抗碱性FGF抗体中和可100%抑制涤纶诱导的有丝分裂原释放，而聚乳糖910诱导的有丝分裂原释放为36%（p < 0.01）。这些结果表明，在体外暴露于生物材料的巨噬细胞中显著诱导有丝分裂原释放，大部分平滑肌细胞有丝分裂原由碱性FGF代表。
Macrophage activation by implanted bood-contacting biomaterials modulates smooth muscle cell and endothelial cell ingrowth. The present study evaluates the in vitro interactions between Dacron or polyglactin 910 with macrophages derived from rabbits fed either normal or atherogenic diets. Peritoneal macrophages were cultured in the presence or absence (negative controls) of either biomaterial for 7 weeks. Conditioned media was evaluated for mitogenic activity using a rabbit aortic smooth muscle cell bioassay with or without preincubation with neutralizing anti-basic-FGF antibody. Results demonstrated increased mitogen release from macrophages harvested from the atherosclerotic rabbits. Only macrophages harvested from normal diet fed rabbits increased their mitogen release following exposure to either polyglactin 910 (p < 0.05) or to Dacron (p < 0.005) over controls. The stimulation of mitogen release by polyglactin 910 did not significantly exceed that in response to Dacron. In rabbits fed normal diets neutralization with the anti-basic-FGF antibody inhibited 100% of the Dacron induced mitogen release as compared to 36% of the polyglactin 910 induced mitogen release (p < 0.01). These results demonstrate significant induced mitogen release from macrophages exposed to biomaterials in vitro, much of the smooth muscle cell mitogen represented by basic-FGF.