Detection of a Few Metallo-Protein Molecules Using Color Centers in Nanodiamonds
Detection of a Few Metallo-Protein Molecules Using Color Centers in Nanodiamonds
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DOI：
10.1021/nl4015233
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发表时间：
2013-07-01
期刊：
影响因子：
10.8
通讯作者：
Jelezko, F.
中科院分区：
文献类型：
--
作者：
Ermakova, A.;Pramanik, G.;Jelezko, F.
含氮空位缺陷中心（NV）的纳米金刚石由于其生物相容性、明亮的荧光和在环境条件下的高磁灵敏度而在生物环境中是有前途的纳米传感器。在这里，我们报告的铁蛋白分子的检测使用的内部顺磁性铁作为对比机制引起的磁噪声。我们观察到由于纳米金刚石表面上存在铁蛋白，相干性和弛豫时间都显著减少。我们的理论模型与实验数据非常吻合，并建立了这种方法作为一种新的传感技术的蛋白质。
Nanometer-sized diamonds containing nitrogen-vacancy defect centers (NV) are promising nanosensors in biological environments due to their biocompatibility, bright fluorescence, and high magnetic sensitivity at ambient conditions. Here we report on the detection of ferritin molecules using magnetic noise induced by the inner paramagnetic iron as a contrast mechanism. We observe a significant reduction of both coherence and relaxation time due to the presence of ferritin on the surface of nanodiamonds. Our theoretical model is in excellent agreement with the experimental data and establishes this method as a novel sensing technology for proteins.