Ontogeny of the dopamine and serotonin transporters in the rat brain: an autoradiographic study
Ontogeny of the dopamine and serotonin transporters in the rat brain: an autoradiographic study
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DOI：
10.1016/j.neulet.2004.04.007
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发表时间：
2004-06-17
影响因子：
2.5
通讯作者：
Chalon, S
中科院分区：
文献类型：
--
作者：
Galineau, L;Kodas, E;Chalon, S
脑发育期间单胺能系统的损伤被认为与几种神经发育障碍有关。我们在一项广泛的研究中，从胚胎期（E-18）到成年期（直到P-70），研究了含有索马和多巴胺能和多巴胺能神经元投射的大鼠大脑区域中多巴胺和5-羟色胺转运体的成熟。使用特定的放射性探针通过定量放射自显影测量膜转运蛋白。我们证明了多巴胺和5-羟色胺转运蛋白具有不同的发育模式。多巴胺转运蛋白密度从E-18增加到P-28，在那里它达到成人水平，然后保持稳定，直到P-70。5-羟色胺转运体的成熟在所有区域都遵循三相曲线：（i）增加导致在细胞体中在P-0和P-14之间以及在P-21在神经末梢中获得峰值;（ii）减少至在P-21在中缝核中以及在P-28在投射区域中达到成人水平;以及（iii）平台期直至P-70。这表明，胚胎生命的最后一周和出生后的前两周是多巴胺能和多巴胺能系统发育的关键时期，此时它们可能特别容易受到损伤。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
Damage to monoaminergic systems during the period of brain development is thought to be involved in several neurodevelopmental disorders. We investigated the maturation of the dopamine and serotonin transporters in rat cerebral regions containing the soma and projections of dopaminergic and serotoninergic neurons in an extensive study from the end of embryonic life (E-18) to adult stages (until P-70). The membrane transporters were measured by quantitative autoradiography using specific radioprobes. We demonstrated that the dopamine and serotonin transporters have different patterns of development. The dopamine transporter density increased from E-18 to P-28 where it reached the adult level and then remained stable until P-70. The maturation of serotonin transporters followed a triphasic profile in all areas: (i) an increase leading to a peak obtained between P-0 and P-14 in cell bodies and at P-21 in nerve endings; (ii) a decrease to reach adult levels at P-21 in raphe nuclei and at P-28 in projections areas; and (iii) a plateau until P-70. This demonstrated that the last week of embryonic life and the first two postnatal weeks are critical periods in the development of the dopaminergic and serotoninergic systems at which time they could be particularly vulnerable to injury. (C) 2004 Elsevier Ireland Ltd. All rights reserved.