NUCLEOTIDE SEQUENCE OF YEAST 5 S RIBOSOMAL-RNA
NUCLEOTIDE SEQUENCE OF YEAST 5 S RIBOSOMAL-RNA
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DOI：
10.1016/0014-5793(72)80563-5
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发表时间：
1972-01-01
期刊：
影响因子：
3.5
通讯作者：
PAGE, SM
中科院分区：
文献类型：
--
作者：
HINDLEY, J;PAGE, SM
在所研究的所有物种的核糖体大亚基中都发现了低分子量的5S核糖体RNA。虽然它在核糖体结构中的功能尚不清楚，但可以预期，它至少在所有不同物种的大亚基的结构和功能中发挥类似的作用。E.大肠杆菌[1]、荧光假单胞菌[2]和KB细胞[3]的5S RNA。物理化学研究[4，5]估计E.大肠杆菌5 S RNA和分子的特定区域对核糖核酸酶T1消化的可变敏感性[6，7]，碳二亚胺修饰[8]和寡核苷酸结合[9]都支持特定的想法，尽管尚未定义，构象。第三个不相关的5 S RNA物种的序列的知识可能会指导结构分析ff一个类似的构象为不同的5 S species assumption.This通信报告的主要序列的卡尔斯伯酵母5 S RNA。该RNA的一个新特征是在5 '-末端出现pppG残基，这使其与所研究的其他5S RNA不同。除非假定细胞中存在激酶，随后使5 '末端磷酸化，否则5'末端三磷酸的存在表明5 S RNA是初级基因产物，而不是来源于在5 '末端具有延伸序列的前体RNA的转录后产物。
The low molecular weight 5 S ribosomal RNA has been found in the larger ribosomal subunit of all species investigated. While its function in ribosome structure is obscure, it would be anticipated that it, at least, fulfills a similar role in the structure and function of the larger subunit of all different species. The primary sequence of the E. coli [1], P. fluorescens [2] and KB-cell [3] 5 S RNA's have been determined. Physico-chemical studies [4, 5] have given estimates of 60-80% base pairing in E. coli 5 S RNA and the variable susceptibility of particular regions of the molecule to digestion with ribonuclease T 1 [6, 7], modification by carbodiimide [8] and oligonucleotide binding [9] all lend support to the idea of a particular, though as yet undefined, conformation. Knowledge of the sequence of a third unrelated 5 S RNA species might be expected to guide structural analysis ff a similar conformation for the different 5 S species is assumed.This communication reports the primary sequence of Saccharomyces carlsbergensis 5 S RNA. A novel feature of this RNA, which distinguishes it from the other 5 S RNA's studied, is the occurrence of a pppG residue at the 5'-terminus. Unless the existence of kinases in the cell, which subsequently phosphorylate the 5'-terminus is assumed, the presence of a 5'-terminal triphosphate suggests the 5 S RNA to be a primary gene product rather than a post-transcriptional product derived from precursor RNA with an extended sequence at the 5'-end.