How to Perform a Nanoindentation Experiment on a Virus
How to Perform a Nanoindentation Experiment on a Virus
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DOI：
10.1007/978-1-61779-282-3_14
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Roos, Wouter H.
中科院分区：
文献类型：
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作者：
Roos, Wouter H.
为了拓宽我们对病毒结构和功能的了解，需要深入了解它们的机械特性。纳米压痕测量与原子力显微镜（AFM）越来越多地被执行探测这样的材料性能。这种单颗粒方法允许确定病毒弹簧常数，它们的杨氏模量，以及发生失效时的力和变形。本文详细介绍了病毒纳米压痕实验的实验过程，重点介绍了表面处理、AFM成像和纳米压痕，以及随后的力距曲线数据分析。尽管AFM可以在空气和液体中操作，但所描述的方法用于探测液体中的单个病毒，以使得能够在生理相关环境中工作。
To broaden our knowledge on virus structure and function, a profound insight into their mechanical properties is required. Nanoindentation measurements with an atomic force microscope (AFM) are increasingly being performed to probe such material properties. This single-particle approach allows for determining the viral spring constant, their Young's modulus, as well as the force and deformation at which failure occurs. The experimental procedures for viral nanoindentation experiments arc described here in detail, focusing on surface preparation, AFM imaging and nanoindentation, and the subsequent data analysis of the force distance curves. Whereas AFM can be operated in air and in liquid, the described methods are for probing single viruses in liquid to enable working in a physiologically relevant environment.