Ragweed pollen collected along high-traffic roads shows a higher allergenicity than pollen sampled in vegetated areas
Ragweed pollen collected along high-traffic roads shows a higher allergenicity than pollen sampled in vegetated areas
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DOI：
10.1111/j.1398-9995.2012.02846.x
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发表时间：
2012-07-01
期刊：
影响因子：
12.4
通讯作者：
Citterio, S.
中科院分区：
文献类型：
--
作者：
Ghiani, A.;Aina, R.;Citterio, S.
背景污染物可能影响花粉过敏性，从而影响过敏的患病率。尽管文献中已有一些研究，但污染与花粉致敏潜力之间的联系尚未明确界定。本研究的目的是通过现场实验评估交通相关污染对豚草（Ambrosia artemisiifolia L.）花粉过敏性的影响。方法从波河平原主要路边和植被区生长的豚草植物中采集成熟花粉粒。取样后立即通过显微镜和图像分析评估亚花粉颗粒释放颗粒（SPPG）的百分比。应用免疫化学和 LC-MS/MS 评估不同花粉样品的整体过敏性和过敏原模式。结果 花粉样品中 SPPG 的百分比没有检测到统计学差异。具体来说，水合后，平均百分比非常低（
Background Pollutants may affect pollen allergenicity and thus the prevalence of allergies. Although a few studies are available in literature, the connection between pollution and the allergenic potential of pollen has yet to be clearly defined. The objective of this study was to evaluate the effect of traffic-related pollution on the allergenicity of ragweed (Ambrosia artemisiifolia L.) pollen through a field-based experiment. Methods Mature pollen grains were collected from ragweed plants grown along main roadsides and in vegetated areas of Po river plain. The percentage of sub-pollen particle-releasing grains (SPPGs) was evaluated immediately after sampling by microscope and image analysis. Immunochemistry and LC-MS/MS were applied to assess the whole allergenicity and the allergen pattern characterizing the different pollen samples. Results No statistical difference was detected in the percentage of SPPGs among pollen samples. Specifically, after hydration, the mean percentage was very low (