Transcriptome of High-Sucrose Sugarcane Variety GT35
Transcriptome of High-Sucrose Sugarcane Variety GT35
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高蔗糖甘蔗品种 GT35 的转录组
DOI：
10.1007/s12355-015-0420-z
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发表时间：
2016-10-01
期刊：
影响因子：
1.9
通讯作者：
Li, Yang-Rui
中科院分区：
文献类型：
--
作者：
Huang, Dong-Liang;Gao, Yi-Jing;Li, Yang-Rui
甘蔗(Saccharum office inarumL.)是最重要的糖类作物，也是生物乙醇的主要来源。蔗糖积累在甘蔗中是重要的，但转录组分析尚未对其进行研究。本研究利用高通量Solexa技术对高蔗糖甘蔗品种GT35的转录组进行了测序。共获得34,105,138个高质量的阅读片段，平均长度为75个碱基对。这些片段被组装成101,255个非基因片段，平均长度为460个碱基，N50长度为640个碱基。BLAST搜索表明，在NCBI非冗余蛋白质序列数据库中有36,472个(36.0%)Uniges具有同源序列，在Swiss-Prot数据库中有23,811个(23.5%)具有同源序列。共有14,613个Uniges被分配了基因本体术语，13,231个被分配了功能注释，并被归类为25个功能类别。对30,756个Uniges的KEGG途径分析揭示了甘蔗转录组中的30多条途径。在3185个非基因组中共检测到3420个简单序列重复序列。本研究丰富了甘蔗转录组资源，为甘蔗基因组与禾本科植物的比较和功能基因组研究奠定了基础，也为进一步阐明甘蔗蔗糖积累规律奠定了基础。
Sugarcane (Saccharum officinarumL.) is the most important sugar crop and a major source of bio-ethanol. Sucrose accumulation is important in sugarcane, but it has not been investigated by transcriptome analysis . In this study, the transcriptome of a high-sucrose sugarcane variety, GT35, was sequenced using high-throughput Solexa technology. A total of 34,105,138 high-quality reads with an average length of 75 bp were obtained. These reads were assembled into 101,255 unigenes, with a mean length of 460 bp and an N50 length of 640 bp. BLAST searches indicated that 36,472 (36.0 %) unigenes had homologous sequences in the NCBI non-redundant protein sequences databases, and 23,811 (23.5 %) had homologous sequences in the Swiss-Prot databases. A total of 14,613 unigenes were assigned gene ontology terms, and 13,231 were assigned functional annotations and grouped into 25 functional categories. A KEGG pathway analysis of 30,756 unigenes revealed more than 30 pathways in the sugarcane transcriptome. A total of 3420 simple sequence repeats were identified in 3185 unigenes. The data in this study enriched the sugarcane transcriptome resources and will be useful for further comparison and functional genomic studies in genusSaccharumand family Poaceae, and will be useful for further detailed elucidation of sucrose accumulation in sugarcane.