Effect of magnesium on ATP-generating and ATP-utilizing reactions in Ehrlich ascites tumour cells.
Effect of magnesium on ATP-generating and ATP-utilizing reactions in Ehrlich ascites tumour cells.
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镁对艾利希腹水肿瘤细胞中 ATP 生成和 ATP 利用反应的影响。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
H. Börnig
中科院分区：
文献类型：
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作者：
K. Schilling;S Preukczas;H. Börnig
用一种在低钙浓度下快速改变细胞内镁含量的方法，研究了镁对艾氏腹水癌细胞能量代谢的影响。失去了部分镁的细胞比未处理的细胞积累乳酸的速度略快。在好氧和厌氧条件下，这些细胞的镁负荷使糖酵解通量降低到20%左右。一些糖酵解代谢产物的相应变化表明，HK-PFK-系统受到抑制。在无葡萄糖和有葡萄糖时，镁对O2-消耗的抑制作用相似，分别约为20%和15%。尽管ATP生成系统受到抑制，但在所研究的所有条件下，ATP浓度都会增加，这表明ATP消耗系统受到了抑制。在镁缺乏和镁负荷的细胞中，哇巴因分别将有氧糖酵解抑制约40%和20%，实验表明，镁影响了活跃的单价阳离子转运。去除镁可以增加(Na+-K+)-ATPase的活性，反之亦然，可能是通过改变细胞膜的通透性来实现的。
The effect of magnesium on the energy metabolism of Ehrlich ascites tumour cells was investigated using a method which allows to change the cellular content of magnesium rapidly at constant low calcium concentration. Cells, which have lost some of their magnesium, accumulate lactate slightly faster than non-treated cells. Mg-loading of these cells decreases the glycolytic flux rates to about 20% under aerobic and anaerobic conditions. The corresponding changes of some glycolytic metabolites suggest an inhibition of the HK-PFK-system. A similar inhibitory effect of Mg on O2-consumption in the absence and presence of glucose to about 20% and 15%, respectively, was observed. Despite the inhibition of the ATP-generating systems the ATP concentration increases under all conditions investigated, indicating an inhibition of ATP consuming systems. From experiments in the presence of ouabain, which inhibits the aerobic glycolysis to about 40% and 20%, in Mg-depleted and Mg-loaded cells, respectively, it is concluded that magnesium affects the active monovalent cation transport. Removal of magnesium increases the activity of the (Na+-K+)-ATPase and vice versa, presumably via changes of the cell membrane permeability.