Blood-Brain Barrier Disruption Mediated by FFA1 Receptor-Evidence Using Miniscope.
Blood-Brain Barrier Disruption Mediated by FFA1 Receptor-Evidence Using Miniscope.
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DOI：
10.3390/ijms23042258
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发表时间：
2022-02-18
影响因子：
5.6
通讯作者：
Brailoiu GC
中科院分区：
文献类型：
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作者：
Lindenau KL;Barr JL;Higgins CR;Sporici KT;Brailoiu E;Brailoiu GC
Omega-3 polyunsaturated fatty acids (n-3 PUFAs), obtained from diet and dietary supplements, have been tested in clinical trials for the prevention or treatment of several diseases. n-3 PUFAs exert their effects by activation of free fatty acid (FFA) receptors. FFA1 receptor, expressed in the pancreas and brain, is activated by medium- to long-chain fatty acids. Despite some beneficial effects on cognition, the effects of n-3 PUFAs on the blood–brain barrier (BBB) are not clearly understood. We examined the effects of FFA1 activation on BBB permeability in vitro, using rat brain microvascular endothelial cells (RBMVEC), and in vivo, by assessing Evans Blue extravasation and by performing live imaging of brain microcirculation in adult rats. AMG837, a synthetic FFA1 agonist, produced a dose-dependent decrease in RBMVEC monolayer resistance assessed with Electric Cell–Substrate Impedance Sensing (ECIS); the effect was attenuated by the FFA1 antagonist, GW1100. Immunofluorescence studies revealed that AMG837 produced a disruption in tight and adherens junction proteins. AMG837 increased Evans Blue content in the rat brain in a dose-dependent manner. Live imaging studies of rat brain microcirculation with miniaturized fluorescence microscopy (miniscope) showed that AMG837 increased extravasation of sodium fluorescein. Taken together, our results demonstrate that FFA1 receptor activation reduced RBMVEC barrier function and produced a transient increase in BBB permeability.
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影响因子：
2.9
作者：
Brailoiu E;Shipsky MM;Yan G;Abood ME;Brailoiu GC
通讯作者：
Brailoiu GC
DOI：
10.1161/atvbaha.112.300943
发表时间：
2013-05-01
影响因子：
8.7
作者：
Honore, Jean-Claude;Kooli, Amna;Chemtob, Sylvain
通讯作者：
Chemtob, Sylvain
影响因子：
5
作者：
Gong, Yuhang;Li, Yanfeng;He, Ling
通讯作者：
He, Ling
影响因子：
6
作者：
Drzazga A;Cichońska E;Koziołkiewicz M;Gendaszewska-Darmach E
通讯作者：
Gendaszewska-Darmach E
DOI：
10.1007/7651_2020_315
发表时间：
2021-01-01
期刊：
PERMEABILITY BARRIER, 2 EDITION
影响因子：
--
作者：
Barr, Jeffrey L.;Brailoiu, G. Cristina;Brailoiu, Eugen
通讯作者：
Brailoiu, Eugen