Burkholderia and Cupriavidus spp. are the preferred symbionts of Mimosa spp. in Southern China
Burkholderia and Cupriavidus spp. are the preferred symbionts of Mimosa spp. in Southern China
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伯克霍尔德氏菌和 Cupriavidus spp.
DOI：
10.1111/j.1574-6941.2012.01310.x
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发表时间：
2012-05-01
影响因子：
4.2
通讯作者：
Wang, Yi Peng
中科院分区：
文献类型：
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作者：
Liu, XiaoYun;Wei, Shuang;Wang, Yi Peng
根瘤菌是从入侵植物含羞草中分离得到的。（M.similar to diplotricha和M.similar to pudica）。98株根瘤菌几乎都是伯克霍尔德氏菌属和贪铜菌属的β-根瘤菌。并与西双版纳地区的菌株进行了比较分析。含每种β-根瘤菌属的根瘤的比例在含羞草属物种之间变化，其中贪铜菌在类似于双丝菌属的根瘤中占优势（63.3%，而伯克霍尔德菌属占36.7%），但类似于普迪卡的根瘤中，伯克霍尔德菌略优于贪铜菌，它们分别占根瘤的56.5%和43.5%。共生必需基因nifA和nifH存在于所有的伯克霍尔德氏菌和贪铜菌菌株测试，和他们的共生基因表明，这些含羞草共生体与本地南美根瘤菌共享共生基因。含羞草属植物16 SrRNA基因、nifH和nifA的进化差异。共生体，表明β-根瘤菌的nod和nif基因是独立进化的。
Rhizobia were isolated from invasive Mimosa spp. (M.similar to diplotricha and M.similar to pudica) in Dehong district of the province of Yunnan in subtropical southern China. Almost all of the 98 isolates were beta-rhizobia in the genera Burkholderia and Cupriavidus. These strains were analysed for their distribution characteristics together with strains from a previous study from Sishuangbanna. The proportion of nodules containing each beta-rhizobial genus varied between Mimosa species, with Cupriavidus being predominant in M.similar to diplotricha nodules (63.3% compared to 36.7% occupation with Burkholderia), but with M.similar to pudica showing a slight preference for Burkholderia over Cupriavidus, with them occupying 56.5% and 43.5% of nodules, respectively. The symbiosis-essential genes nodA and nifH were present in all the Burkholderia and Cupriavidus strains tested, and their phylogenies indicated that these Mimosa symbionts share symbiotic genes with native South American rhizobia. The evolutionary discrepancies among 16S rRNA genes, nodA and nifH of Mimosa spp. symbionts, suggests that the nod and nif genes of beta-rhizobia evolved independently.