The re-expression of two myosin heavy chains in regenerated rat muscle spindles.
The re-expression of two myosin heavy chains in regenerated rat muscle spindles.
复制标题
再生大鼠肌梭中两条肌球蛋白重链的重新表达。
DOI：
10.1016/0304-3940(89)90142-0
复制
发表时间：
1989
影响因子：
2.5
通讯作者：
Walro,JM
中科院分区：
文献类型：
--
作者：
Fei,C;Walro,JM
将两只成年大鼠趾长伸肌（EDL）的血管供应和肌腱单侧切断，并使肌肉再生40天。连续冷冻切片的肌肉移植物进行切割和染色的酶描绘的纤维类型，感觉末梢和运动末梢。MF30和ALD58，两种仅与正常大鼠肌肉中的梭内纤维反应的抗体，与神经完整的肌肉移植物的切片反应。数据与正常大鼠的肌肉进行比较。肌移植物中缺乏感觉神经支配的包裹纤维和一些梭外纤维对MF 30有弱至中度反应，但对ALD 58无反应。再生的，封装的感觉神经支配纤维绑定MF30和ALD58。这些数据表明，传入神经支配再生纺锤体保留的能力，诱导表达的纺锤体特异性肌球蛋白亚型在大鼠。
The vascular supply and tendons of the extensor digitorum longus (EDL) muscles of two adult rats were unilaterally severed and the muscles were allowed to regenerate for 40 days. Serial frozen sections of muscle grafts were cut and stained for enzymes that delineated fiber type, sensory endings and motor endings. MF30 and ALD58, two antibodies which react only to intrafusal fibers in normal rat muscle, were reacted against sections of nerve-intact muscle grafts. Data were compared to that from muscles of normal rats. Encapsulated fibers devoid of sensory innervation and some extrafusal fibers in muscle grafts had a weak to moderate reaction to MF30, but no reaction to ALD58. Regenerated, encapsulated fibers with sensory innervation bound both MF30 and ALD58. These data indicate that afferents which reinnervate regenerated spindles retain the capacity to induce expression of spindle-specific myosin isoforms in rats.