Changes of alternative splicing in Arabidopsis thaliana grown under different CO2 concentrations
Changes of alternative splicing in Arabidopsis thaliana grown under different CO2 concentrations
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不同CO2浓度下拟南芥选择性剪接的变化
DOI：
10.1016/j.gene.2018.11.083
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发表时间：
2019-03-20
期刊：
影响因子：
3.5
通讯作者：
Tian, Jingkui
中科院分区：
文献类型：
--
作者：
Huang, Wei;Chen, Xi;Tian, Jingkui
大气CO2浓度是影响植物生长和作物产量的重要因素之一。虽然许多关键基因和途径已被确定在响应大气CO2变化，完整的和精确的机制，植物CO2响应还没有得到很好的理解。选择性剪接（alternative splicing，AS）是一种重要的基因调控过程，影响着植物的许多生物学过程.然而，AS模式的变化在植物中响应CO（2-l）水平升高尚未调查。在这里，我们使用不同CO2浓度下生长的拟南芥的RNA-Seq数据来分析AS的全球变化。我们发现AS随着CO2浓度的增加而增加。此外，我们还发现了345个差异表达（DE）基因和251个差异选择性剪接（DAS）基因在高CO2条件下。此外，结果表明，大多数DAS基因的表达没有显着变化，表明AS可以作为一个独立的机制，基因调控响应CO2浓度升高。此外，我们的功能类别的分析表明，DAS基因主要与刺激反应。总的来说，这是第一个研究探讨AS在植物中响应CO2浓度升高的变化。
Atmospheric CO2 level is one of the most important factors which affect plant growth and crop production. Although many crucial genes and pathways have been identified in response to atmospheric CO2 changes, the integrated and precise mechanisms of plant CO2 response are not well understood. Alternative splicing (AS) is an important gene regulation process that affects many biological processes in plants. However, the AS pattern changes in plants in response to elevated CO(2 l)evels have not yet been investigated. Here, we used RNA-Seq data of Arabidopsis thaliana grown under different CO2 concentration to analyze the global changes in AS. We found that AS increased with the rise in CO2 concentration. Additionally, we identified 345 differentially expressed (DE) genes and 251 differentially alternative splicing (DAS) genes under the elevated CO2 condition. Moreover, the results showed that the expression of most of the DAS genes did not change significantly, indicating that AS can serve as an independent mechanism for gene regulation in response to elevated CO2. Furthermore, our analysis of function categories revealed that the DAS genes were associated mainly with the stimulus response. Overall, this the first study to explore the changes of AS in plants in response to elevated CO2.