Generic Method of Preparing Multifunctional Fluorescent Nanoparticles Using Flash NanoPrecipitation
Generic Method of Preparing Multifunctional Fluorescent Nanoparticles Using Flash NanoPrecipitation
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DOI：
10.1002/adfm.200801583
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发表时间：
2009-03-10
影响因子：
19
通讯作者：
Prud'homme, Robert K.
中科院分区：
文献类型：
--
作者：
Akbulut, Mustafa;Ginart, Paul;Prud'homme, Robert K.
对具有高荧光产率的纳米颗粒的需求增加，所述纳米颗粒具有所需的激发波长、表面官能化和粒度以用作生物探针。在这里，一个简单，快速，和强大的方法，快速纳米沉淀（FNP），以产生这样的荧光纳米粒子的描述。该过程涉及在动力学冷冻状态下用两亲性生物相容性二嵌段共聚物包封疏水性荧光团。FNP用于产生范围为30至800 nm的纳米颗粒，其中荧光发射峰范围为但不限于370 nm至720 nm。这种荧光纳米颗粒在水溶液中保持稳定，并且与可溶性染料相反，没有显示出光漂白。荧光团和药物被整合到单个纳米颗粒中，允许同时进行药物递送和生物成像。此外，纳米颗粒表面与疾病特异性配体的功能化允许精确的细胞靶向。这些特征使得FNP产生的荧光纳米颗粒对于各种生物应用非常理想。
There is increased demand for nanoparticles with a high fluorescence yield that have the desired excitation wavelength, surface functionalization, and particle size to act as biological probes. Here, a simple, rapid, and robust method, Flash NanoPrecipitation (FNP), to produce such fluorescent nanoparticles is described. This process involves encapsulation of a hydrophobic fluorophore with an amphiphilic biocompatible diblock copolymer in a kinetically frozen state. FNP is used to produce nanoparticles ranging from 30 to 800 nm with fluorescence emission peaks ranging from, but not limited to, 370 nm to 720 nm. Such fluorescent nanoparticles remain stable in aqueous solutions, and, in contrast to soluble dyes, show no photobleaching. Fluorophores and drugs are incorporated into a single nanoparticle, allowing for simultaneous drug delivery and biological imaging. In addition, functionalization of nanoparticle surfaces with disease-specific ligands permits precise cell targeting. These features make FNP-produced fluorescent nanoparticles highly desirable for various biological applications.