Expression pattern of a mini human PrP gene promoter in transgenic mice
Expression pattern of a mini human PrP gene promoter in transgenic mice
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DOI：
10.1006/nbdi.2002.0486
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发表时间：
2002-06-01
影响因子：
6.1
通讯作者：
Collinge, J
中科院分区：
文献类型：
--
作者：
Asante, EA;Gowland, I;Collinge, J
朊病毒蛋白是朊病毒疾病发病机制的核心，尽管其确切功能尚不清楚。虽然转基因小鼠已被广泛用于朊病毒的研究，其PrP的表达模式还没有详细的特点。我们研究了一个214 bp的微型人PrP启动子在转基因小鼠中的发育时空表达。转基因表达在胚胎第12.5天首次被检测到，这比先前通过原位杂交对内源性小鼠基因的报道早一天。一般的表达模式密切反映了内源性小鼠PrP基因的表达模式，使得这种小且明确定义的转基因盒可以取代使用基于大粘粒的载体用于人类和动物朊病毒疾病的转基因建模的需要。(C)2002 Elsevier Science（美国）。
The prion protein is central to the pathogenesis of prion diseases, although its exact function remains unclear. Although transgenic mice have been widely utilised in prion research, their PrP expression patterns have not been characterised in detail. We have studied the developmental temporal and spatial expression of a 214-bp mini human PrP promoter in transgenic mice. Transgene expression is first detected at embryonic day 12.5, a day earlier than previously reported for endogenous mouse gene by in situ hybridization. The general expression pattern closely mirrors that of the endogenous mouse PrP gene, such that this small and clearly defined transgene cassette can replace the need to use large cosmid based vectors for transgenetic modeling of human and animal prion disease. (C) 2002 Elsevier Science (USA).