Mass Spectrometry Analysis of Photo-Induced Methionine Oxidation of a Recombinant Human Monoclonal Antibody
Mass Spectrometry Analysis of Photo-Induced Methionine Oxidation of a Recombinant Human Monoclonal Antibody
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DOI：
10.1016/j.jasms.2008.11.011
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发表时间：
2009-03-01
影响因子：
3.2
通讯作者：
Zhou, Lisa
中科院分区：
文献类型：
--
作者：
Liu, Hongcheng;Gaza-Bulseco, Georgeen;Zhou, Lisa
研究了重组人单抗的蛋氨酸(Met)残基在光照下的氧化，并与测试丁基过氧化氢(TBHP)化学诱导的氧化进行了比较。光照或与tBHP孵育的样品中Fc区的Met256和Met432被氧化。在光照条件下，抗体的Fc谱以峰为主，分子量增加32Da，而经tBHP处理的抗体峰仅增加16Da。这些结果表明，当抗体暴露在光照下时，一个Met残基(Met256或Met432)的氧化催化同一重链(HC)上的第二个Met残基的氧化，或者一个HC的Met残基优先被氧化，而当抗体与tBHP孵育时，Met256和Met432被随机氧化。(J am Soc质谱学2009，20,525-528)(C)2009美国质谱学学会
Oxidation of methionine (Met) residues of a recombinant fully human monoclonal antibody after exposure to light was investigated and compared with chemically induced oxidation using test-butyl-hydroperoxide (tBHP). Met256 and Met432 in the Fc region in the samples exposed to light or incubated with tBHP were oxidized. The Fc mass spectra of the antibody exposed to light showed mainly peaks with a molecular weight (MW) increase of 32 Da, however the sample treated with tBHP showed peaks with increase of only 16 Da. These results suggested that either oxidation of one Met residue (either Met256 or Met432) catalyzed the oxidation of the second Met residue on the same heavy chain (HC) or Met residues of one HC were preferentially oxidized when the antibody was exposed to light, while Met256 and Met432 were randomly oxidized when the antibody was incubated with tBHP. (J Am Soc Mass Spectrom 2009, 20, 525-528) (C) 2009 American Society for Mass Spectrometry