Reproduction of Meloidogyne javanica on Plant Roots Genetically Transformed by Agrobacterium rhizogenes.
Reproduction of Meloidogyne javanica on Plant Roots Genetically Transformed by Agrobacterium rhizogenes.
复制标题
爪哇根结线虫在发根农杆菌遗传转化的植物根上的繁殖。
作者：
S. Verdejo;B. Jaffee;R. Mankau
在单细胞条件下，对几种发根农杆菌转化的根培养物上的爪哇根结线虫的繁殖进行了比较。 M. javanica 在所有测试的转化根上繁殖；然而，马铃薯和南澳大利亚早矮红番茄比旋花、热带番茄、利马豆或胡萝卜获得更多雌虫和卵。以中等速率生长到琼脂中并产生许多次生根的根支持最高的繁殖。转化马铃薯根培养物中产生的雌性数量随着线虫接种水平的增加而增加，无论接种物是分散的卵还是幼体。雌性看起来更小，产生的卵更少，并且在较高的接种水平下发现了合并的虫瘿。随着幼体接种量的增加，最终种群与初始种群之间的比率急剧下降。第二阶段的幼体比分散的卵或卵块更适合用于组织培养系统的接种，因为接种物的数量和活力易于评估。用转化的根培养物培育的爪哇根结线虫能够在新的转化根培养物或温室番茄植物上完成其生命周期。
Reproduction of Meloidogyne javanica was compared on several Agrobacterium rhizogenes-transformed root cultures under monoxenic conditions. M. javanica reproduced on all transformed roots tested; however, more females and eggs were obtained on potato and South Australian Early Dwarf Red tomato than on bindweed, Tropic tomato, lima bean, or carrot. Roots that grew at moderate rates into the agar and produced many secondary roots supported the highest reproduction. Numbers of females produced in cultures of transformed potato roots increased with increasing nematode inoculum levels, whether inoculum was dispersed eggs or juveniles. Females appeared smaller, produced fewer eggs, and were found in coalesced galls at the higher inoculum levels. The ratio between the final and initial population decreased sharply as the juvenile inoculum increased. The second-stage juvenile was preferred to dispersed eggs or egg masses for inoculation of tissue culture systems because quantity and viability of inoculum were easily assessed. Meloidogyne javanica reared on transformed root cultures were able to complete their life cycles on new transformed root cultures or greenhouse tomato plants.