An epidermal neural crest stem cell (EPI-NCSC) molecular signature
An epidermal neural crest stem cell (EPI-NCSC) molecular signature
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DOI：
10.1634/stemcells.2006-0233
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发表时间：
2006-12-01
期刊：
影响因子：
5.2
通讯作者：
Sieber-Blum, Maya
中科院分区：
文献类型：
--
作者：
Hu, Yao Fei;Zhang, Zhi-Jian;Sieber-Blum, Maya
在这里，我们报告了小鼠表皮神经嵴干细胞（EPI-NCSC，以前的 eNCSC）的第一个转录组。此外，我们的研究通过表明 EPI-NCSC 与其他类型的皮肤驻留干细胞/祖细胞不同，解决了文献中相互矛盾的观点。最后，通过这三个基因图谱，我们为未来的小鼠 NCSC 研究奠定了基础并提供了宝贵的资源。 EPI-NCSC 代表一种新型多能成体干细胞，起源于胚胎神经嵴并驻留在毛囊的隆起处。我们通过 LongSAGE（基因表达的长期连续分析）对来自 EPI-NCSC、胚胎 NCSC 和体外分化的胚胎神经嵴后代的 mRNA 进行了基因分析。我们已经鉴定了重要的差异表达基因，包括新基因和疾病基因。此外，使用严格的标准，我们定义了一个 NCSC 分子特征，它由一组 19 个基因组成，代表 EPI-NCSC 和 NCSC。 EPI-NCSC具有结合胚胎干细胞和成体干细胞优点的特点。与胚胎干细胞类似，EPI-NCSC具有高度的先天可塑性，可以高纯度分离，并且可以在体外扩增。与其他类型的成体干细胞类似，EPI-NCSC 可以通过微创手术轻松获得。多能成年哺乳动物干细胞引起了极大的兴趣，因为它们在未来通过自体移植进行的细胞替代疗法中具有潜在价值，可避免移植排斥。
Here, we report the first transcriptome for mouse epidermal neural crest stem cells (EPI-NCSC, formerly eNCSCs). In addition, our study resolves conflicting opinions in the literature by showing that EPI-NCSC are distinct from other types of skin-resident stem cells/progenitors. Finally, with the three gene profiles, we have established a foundation and provide a valuable resource for future mouse NCSC research. EPI-NCSC represent a novel type of multipotent adult stem cell that originates from the embryonic neural crest and resides in the bulge of hair follicles. We performed gene profiling by LongSAGE ( long serial analysis of gene expression) with mRNA from EPI-NCSC, embryonic NCSC, and in vitro differentiated embryonic neural crest progeny. We have identified important differentially expressed genes, including novel genes and disease genes. Furthermore, using stringent criteria, we have defined an NCSC molecular signature that consists of a panel of 19 genes and is representative of both EPI-NCSC and NCSC. EPI-NCSC have characteristics that combine advantages of embryonic and adult stem cells. Similar to embryonic stem cells, EPI-NCSC have a high degree of innate plasticity, they can be isolated at high levels of purity, and they can be expanded in vitro. Similar to other types of adult stem cell, EPI-NCSC are readily accessible by minimal invasive procedure. Multipotent adult mammalian stem cells are of great interest because of their potential value in future cell replacement therapy by autologous transplantation, which avoids graft rejection.