Effect of aperture number on pollen germination, survival and reproductive success in Arabidopsis thaliana
Effect of aperture number on pollen germination, survival and reproductive success in Arabidopsis thaliana
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DOI：
10.1093/aob/mcx206
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发表时间：
2018-03-01
期刊：
影响因子：
4.2
通讯作者：
Dobritsa, Anna A.
中科院分区：
文献类型：
--
作者：
Albert, Beatrice;Ressayre, Adrienne;Dobritsa, Anna A.
开花植物的花粉粒显示出迷人的多样性的形式，包括不同图案的孔，花粉表面上的专门区域，通常作为花粉管起始的位点，因此，可能在生殖中发挥关键作用。虽然被子植物中存在许多孔模式，但三孔花粉构成了真双子叶植物中发现的主要花粉类型。本研究以拟南芥（Arabidopsis thaliana）花粉为材料，比较了不同萌发孔数的花粉在活体条件下的萌发、花粉管生长、寿命和结实能力，探讨了三孔花粉在真双子叶植物花粉存活、萌发和繁殖成功率方面的选择优势。为此，使用了拟南芥花粉孔系列，其中包括三孔野生型，以及突变体没有孔（inaperturate）和具有三个以上的孔。孔数似乎影响花粉粒性能。在大多数萌发和寿命实验中，三孔和无孔花粉粒的表现优于具有较高孔径数的花粉粒。在混合授粉中，三孔花粉和无孔花粉被迫相互竞争，三孔花粉优于无孔花粉，三孔花粉可能提供了各种花粉性能性状之间的最佳平衡，从而解释了这种形态特征在真双子叶植物分支中的普遍存在。
Pollen grains of flowering plants display a fascinating diversity of forms, including diverse patterns of apertures, the specialized areas on the pollen surface that commonly serve as the sites of pollen tube initiation and, therefore, might play a key role in reproduction. Although many aperture patterns exist in angiosperms, pollen with three apertures (triaperturate) constitutes the predominant pollen type found in eudicot species. The aim of this study was to explore whether having three apertures provides selective advantages over other aperture patterns in terms of pollen survival, germination and reproductive success, which could potentially explain the prevalence of triaperturate pollen among eudicots.The in vivo pollen germination, pollen tube growth, longevity and competitive ability to sire seeds were compared among pollen grains of Arabidopsis thaliana with different aperture numbers. For this, an arabidopsis pollen aperture series was used, which included the triaperturate wild type, as well as mutants without an aperture (inaperturate) and with more than three apertures.Aperture number appears to influence pollen grain performance. In most germination and longevity experiments, the triaperturate and inaperturate pollen grains performed better than pollen with higher aperture numbers. In mixed pollinations, in which triaperturate and inaperturate pollen were forced to compete with each other, the triaperturate pollen outperformed the inaperturate pollen.Triaperturate pollen grains might provide the best trade-off among various pollen performance traits, thus explaining the prevalence of this morphological trait in the eudicot clade.