One-Pot Synthesis of Near-Infrared Fluorescent Gold Clusters for Cellular Fluorescence Lifetime Imaging
One-Pot Synthesis of Near-Infrared Fluorescent Gold Clusters for Cellular Fluorescence Lifetime Imaging
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DOI：
10.1002/smll.201100746
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发表时间：
2011-09-19
期刊：
影响因子：
13.3
通讯作者：
Nienhaus, G. Ulrich
中科院分区：
文献类型：
--
作者：
Shang, Li;Azadfar, Naghmeh;Nienhaus, G. Ulrich
报道了一种合成用双齿配体二氢硫辛酸（DHLA）稳定的水溶性荧光金纳米团簇（Au NC）的简便策略。 DHLA 封端的 Au NC 通过紫外可见吸收光谱、荧光光谱、透射电子显微镜和 X 射线光电子能谱进行表征。 Au NCs 具有许多吸引人的特性，包括超小尺寸、明亮的近红外发光、高胶体稳定性和良好的生物相容性，使其成为生物医学和细胞成像应用的有前途的成像剂。此外，它们的长荧光寿命（> 100 ns）使其作为荧光寿命成像（FLIM）应用中的标签具有吸引力。例如，使用 FLIM 技术可视化活 HeLa 细胞对 Au NC 的内化。
A facile strategy to synthesize water-soluble fluorescent gold nanoclusters (Au NCs) stabilized with the bidentate ligand dihydrolipoic acid (DHLA) is reported. The DHLA-capped Au NCs are characterized by UV-vis absorption spectroscopy, fluorescence spectroscopy, transmission electron microscopy, and X-ray photoelectron spectroscopy. The Au NCs possess many attractive features including ultrasmall size, bright near-infrared luminescence, high colloidal stability, and good biocompatibility, making them promising imaging agents for biomedical and cellular imaging applications. Moreover, their long fluorescence lifetime (>100 ns) makes them attractive as labels in fluorescence lifetime imaging (FLIM) applications. As an example, the internalization of Au NCs by live HeLa cells is visualized using the FLIM technique.