Expression of inducible nitric oxide (NO) synthase but not prevention by its gene ablation of hepatocarcinogenesis with fibrosis caused by a choline-deficient, L-amino acid-defined diet in rats and mice
Expression of inducible nitric oxide (NO) synthase but not prevention by its gene ablation of hepatocarcinogenesis with fibrosis caused by a choline-deficient, L-amino acid-defined diet in rats and mice
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DOI：
10.1016/j.niox.2006.07.002
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发表时间：
2007-02-01
影响因子：
3.9
通讯作者：
Kuniyasu, Hiroki
中科院分区：
文献类型：
--
作者：
Denda, Ayumi;Kitayama, Wakashi;Kuniyasu, Hiroki
在雄性F344大鼠和C57 BL/6 J野生型和iNOS-/-小鼠中检测了诱导型一氧化氮合酶（iNOS）的表达和iNOS基因切除对胆碱缺乏、L-氨基酸限定（CDAA）饮食引起的纤维化相关肝癌发生的影响。Western blot、RT-PCR和免疫组化分析显示，喂食CDAA饮食12-80周的大鼠和野生型小鼠肝脏中iNOS蛋白和mRNA的表达增加，与血清NOx和肝脏硝基酪氨酸水平升高相关。iNOS-/-小鼠表现出更大的肝损伤和纤维化的早期阶段比野生型的同行，但这并没有显着影响的发病率和多样性的改变灶，腺瘤和肝细胞癌，尽管在这些病变的免疫组化iNOS表达。结果表明，在CDAA饮食引起的肝肿瘤的发展中，表达的iNOS没有主要的决定性作用。(c)2006年爱思唯尔公司All rights reserved.
Expression of inducible nitric oxide synthase (iNOS) and effects of iNOS gene ablation on the hepatocarcinogenesis associated with fibrosis caused by a choline-deficient, L-amino acid-defined (CDAA) diet, were examined in male F344 rats and C57BL/6J wild-type and iNOS-/- mice. Western blot, RT-PCR and immunohistochemical analyses revealed increased expression of iNOS protein and mRNA in the livers of rats and wild-type mice fed a CDAA diet for 12-80 weeks, associated with elevated serum NOx and liver nitrotyrosine levels. iNOS-/- mice demonstrated greater liver injury and fibrosis in the early stage than their wild-type counterparts, but this did not significantly affect the incidence and multiplicity of altered foci, adenomas and hepatocellular carcinomas in spite of immunohistochemical iNOS expression in these lesions. Results suggested no major determinant roles of the expressed iNOS in the development of liver tumors caused by the CDAA diet. (c) 2006 Elsevier Inc. All rights reserved.