Developmentally controlled farnesylation modulates AtNAP1;1 function in cell proliferation and cell expansion during Arabidopsis leaf development
Developmentally controlled farnesylation modulates AtNAP1;1 function in cell proliferation and cell expansion during Arabidopsis leaf development
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DOI：
10.1104/pp.106.088344
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发表时间：
2006-12-01
期刊：
影响因子：
7.4
通讯作者：
Gruissem, Wilhelm
中科院分区：
文献类型：
--
作者：
Galichet, Arnaud;Gruissem, Wilhelm
在多细胞生物中，器官发生需要细胞增殖、细胞扩增和细胞分化的严格控制和协调。我们已经确定了拟南芥（拟南芥）核小体组装蛋白1（AtNAP 1;1）作为一个组成部分的调节机制，连接细胞增殖，细胞生长和扩展在拟南芥叶发育。分子，生物化学和动力学研究AtNAP 1; 1获得或丧失功能的突变体表明，AtNAP 1; 1促进细胞增殖或细胞扩增的发展背景下，作为一个功能的法尼基化状态的蛋白质。AtNAP 1; 1是法尼基化和定位到细胞核在细胞增殖阶段的叶发育，当它促进细胞分裂。在叶发育后期，nonfarnesylated AtNAP 1; 1积累在细胞质中，当它促进细胞扩张。异位表达的nonfarnesylated AtNAP 1; 1，定位于细胞质，破坏了这一发展计划，促进计划外的细胞扩增在增殖阶段。
In multicellular organisms, organogenesis requires tight control and coordination of cell proliferation, cell expansion, and cell differentiation. We have identified Arabidopsis ( Arabidopsis thaliana) nucleosome assembly protein 1 (AtNAP1;1) as a component of a regulatory mechanism that connects cell proliferation to cell growth and expansion during Arabidopsis leaf development. Molecular, biochemical, and kinetic studies of AtNAP1; 1 gain- or loss-of-function mutants indicate that AtNAP1; 1 promotes cell proliferation or cell expansion in a developmental context and as a function of the farnesylation status of the protein. AtNAP1; 1 was farnesylated and localized to the nucleus during the cell proliferation phase of leaf development when it promotes cell division. Later in leaf development, nonfarnesylated AtNAP1; 1 accumulates in the cytoplasm when it promotes cell expansion. Ectopic expression of nonfarnesylated AtNAP1; 1, which localized to the cytoplasm, disrupts this developmental program by promoting unscheduled cell expansion during the proliferation phase.