Chitinolytic enzymes: An appraisal as a product of commercial potential
Chitinolytic enzymes: An appraisal as a product of commercial potential
复制标题
DOI：
10.1002/btpr.1732
复制
发表时间：
2013-07-01
影响因子：
2.9
通讯作者：
Deshpande, M. V.
中科院分区：
文献类型：
--
作者：
Chavan, S. B.;Deshpande, M. V.
甲壳素、脱乙酰甲壳素、壳聚糖和几丁质降解酶，即内切几丁质酶、N-乙酰氨基葡萄糖苷酶、壳聚糖酶、甲壳素脱乙酰酶（CDA）在生物技术中的应用越来越重要。目前，甲壳素降解酶在人类保健和相关研究中是高成本低产量的产品。事实上，几丁质酶和CDA-壳聚糖酶复合物在农业上控制植物病原真菌和昆虫方面具有巨大的潜力。探索几丁质酶特别是用于农业的几丁质酶的成功，即作为一种高容量低成本产品，取决于具有合理成本的高活性制剂的可用性。因此，重新考虑理解几丁质分解酶在应用中的作用，例如用于生物防治的宿主-病原体相互作用，几丁质降解的不同机制，以及具有不同特异性的新酶的鉴定，可能使它们在不久的将来在各种商业过程中更有用。在将概念验证转化为商业产品过程中可能遇到的问题和挑战将在本综述中进行评估。(c)2013年美国化学工程师学会生物技术。程序，29：833-846，2013
Chitin, its deacetylated form, chitosan and chitinolytic enzymes viz. endo-chitinase, N-acetylglucosaminidase, chitosanase, chitin deacetylase (CDA) are gaining importance for their biotechnological applications. Presently, chitin degrading enzymes constitute high-cost low-volume products in human health care and associated research. Indeed chitinases and CDA-chitosanase complex possesss tremendous potential in agriculture to control plant pathogenic fungi and insects. The success in exploring chitinases especially for agriculture, i.e. as a high-volume low-cost product, depends on the availability of highly active preparations with a reasonable cost. Therefore, a reconsideration in terms of understanding the roles of chitinolytic enzymes in applications, e.g. host-pathogen interaction for biocontrol, different mechanisms of chitin degradation, and identification of new enzymes with varying specificities, may make them more useful in a variety of commercial processes in the near future. The possible issues and challenges encountered in the translation of proof of concept into a commercial product will be appraised in this review. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:833-846, 2013