Osmotic surveillance mediates rapid wound closure through nucleotide release
Osmotic surveillance mediates rapid wound closure through nucleotide release
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DOI：
10.1083/jcb.201408049
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发表时间：
2014-12-22
影响因子：
7.8
通讯作者：
Niethammer, Philipp
中科院分区：
文献类型：
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作者：
Gault, William J.;Enyedi, Balazs;Niethammer, Philipp
来自环境的渗透提示介导的幼斑马鱼尾鳍中白细胞对上皮破裂的快速检测。使用活体发光和荧光显微镜，我们现在表明，渗透压之间的差异，间质液和外部环境触发ATP释放在尾鳍伤口启动快速伤口闭合通过远程激活的基础上皮细胞的运动。细胞外核苷酸降解，至少部分由外三磷酸核苷二磷酸水解酶3（Entpd3）介导，限制了损伤后免疫诱导细胞迁移的范围和持续时间。因此，在斑马鱼幼体中，伤口修复是由自动调节电路驱动的，该自动调节电路根据组织内部的环境暴露产生促迁移组织信号。
Osmotic cues from the environment mediate rapid detection of epithelial breaches by leukocytes in larval zebrafish tail fins. Using intravital luminescence and fluorescence microscopy, we now show that osmolarity differences between the interstitial fluid and the external environment trigger ATP release at tail fin wounds to initiate rapid wound closure through long-range activation of basal epithelial cell motility. Extracellular nucleotide breakdown, at least in part mediated by ecto-nucleoside triphosphate diphosphohydrolase 3 (Entpd3), restricts the range and duration of osmotically induced cell migration after injury. Thus, in zebrafish larvae, wound repair is driven by an autoregulatory circuit that generates pro-migratory tissue signals as a function of environmental exposure of the inside of the tissue.