Rapid direct detection of the major fish allergen, parvalbumin, by selected MS/MS ion monitoring mass spectrometry
Rapid direct detection of the major fish allergen, parvalbumin, by selected MS/MS ion monitoring mass spectrometry
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DOI：
10.1016/j.jprot.2012.03.030
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发表时间：
2012-06-18
影响因子：
3.3
通讯作者：
Gallardo, Jose M.
中科院分区：
文献类型：
--
作者：
Carrera, Monica;Canas, Benito;Gallardo, Jose M.
小白蛋白（β - prvbs）被认为是主要的鱼类过敏原。本文提出了一种快速直接检测食品中过敏原的新方法。所提出的方法是基于加热纯化β - prvb，在高强度聚焦超声（HIFU）提供的超声场下使用加速溶液胰蛋白酶消化，以及在线性离子阱（LIT）质谱仪中使用选择的MS/MS离子监测（SMIM）监测19个β - prvb肽生物标志物。目前的策略允许在不到2小时的时间内直接检测任何食品中鱼β - prvbs的存在。(C) 2012 Elsevier B.V.版权所有
Parvalbumins beta (beta-PRVBs) are considered the major fish allergens. A new strategy for the rapid and direct detection of these allergens in any foodstuff is presented in this work. The proposed methodology is based on the purification of beta-PRVBs by treatment with heat, the use of accelerated in-solution trypsin digestion under an ultrasonic field provided by High-Intensity Focused Ultrasound (HIFU) and the monitoring of only nineteen beta-PRVB peptide biomarkers by Selected MS/MS Ion Monitoring (SMIM) in a linear ion trap (LIT) mass spectrometer. The present strategy allows the direct detection of the presence of fish beta-PRVBs in any food product in less than 2 hours. (C) 2012 Elsevier B.V. All rights reserved.