Gastric and Intestinal Phenotypic Expression of Human Stomach Cancers as Revealed by Pepsinogen lmmunohistochemistry and Mucin Histochemistry
Gastric and Intestinal Phenotypic Expression of Human Stomach Cancers as Revealed by Pepsinogen lmmunohistochemistry and Mucin Histochemistry
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胃蛋白酶原免疫组织化学和粘蛋白组织化学揭示人胃癌的胃和肠道表型表达
DOI：
10.1111/j.1440-1827.1990.tb01591.x
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发表时间：
1990
影响因子：
2.2
通讯作者：
N. Ito
中科院分区：
文献类型：
--
作者：
M. Tatematsu;M. Ichinose;K. Miki;R. Hasegawa;T. Kato;N. Ito
采用粘蛋白组织化学和胃蛋白酶原（Pg）免疫组织化学方法研究了223例手术获得的原发性胃癌患者的胃和肠道表型表达及其与胃黏膜周围肠化生的组织发生关系。粘蛋白的组织化学分化（矛盾的魔芋豆蛋白A，半乳糖氧化酶-希夫序列和唾液酸酶-半乳糖氧化酶希夫序列）和Pgs I和II的免疫组化染色，使不同组织学类别的胃癌细胞分化为胃成分，包括粘膜颈细胞、幽门腺细胞和表面粘膜细胞，或肠成分，包括杯状细胞和肠吸收细胞类型。122例乳头状和管状腺癌中，33例（27.1%）以胃型细胞为主，42例（34.4%）以肠型细胞为主。其余（38.5%）由胃型和肠型细胞混合组成。101例低分化腺癌、印戒细胞癌和粘液腺癌中，59例（58.4%）以胃型细胞为主，20例（19.8%）以肠型细胞为主。35例以胃型癌细胞为主的乳头状和管状腺癌中有7例被肠上皮化生的粘膜包围。相反，在40个主要由肠型癌细胞组成的乳头状和管状腺癌中，有10个在未化生的胃粘膜中观察到。由此可见，胃癌与胃周围粘膜在肠道表型表达方面没有相关性。
Gastric and intestinal phenotypic expression in 223 surgically obtained primary gastric cancers and their histogenetic relationship to intestinal metaplasia in the surrounding gastric mucosa were studied by mucin histochemistry and pepsinogen (Pg) immunohistochemistry. Histochemical differentiation of mucins (paradoxical concanavalin A, the galactose oxidase‐Schiff sequence and sialidase galactose oxidase Schiff) and immunohisto chemical staining of Pgs I and II, allowed differentiation of gastric cancer cells from different histological categories into gastric elements including mucous neck cells, pyloric gland cells and surface mucous cells or intestinal elements including goblet cell and intestinal absorptive cell types. Of 122 papillary and tubular adenocarcinomas, 33 (27.1%) consisted mainly of gastric type cells and 42 (34.4%) predominantly of intestinal type cells. The remainder (38.5%) consisted of mixtures of gastric‐ and intestinal‐type cells. Of 101 poorly differentiated adenocarcinomas, signet ring cell carcinomas and mucinous adenocarcinomas, 59 (58.4%) consisted mainly of gastric‐type cells and 20 (19.8%) mainly of intestinal‐type cells. Seven out of 35 papillary and tubular adenocarcinomas consisting mainly of gastric type cancer cells were surrounded by mucosa with intestinal metaplasia. Conversely, 10 out of 40 papillary and tubular adenocarcinomas consisting mainly of intestinal‐type cancer cells were observed in non metaplastic gastric mucosa. Thus no relationship as regards intestinal phenotypic expression was found between gastric cancers and surrounding gastric mucosa.
胃癌中的胃蛋白酶原 I 和 II：一项免疫组织化学研究。
DOI：
--
发表时间：
1985
期刊：
Applied pathology
影响因子：
--
作者：
Stemmermann,GN;Samloff,IM;Hayashi,T
通讯作者：
Hayashi,T
使用凝集素-辣根过氧化物酶缀合物和半乳糖氧化酶-希夫序列对啮齿动物和人类气管腺分泌糖蛋白中的糖残基进行光学显微镜组织化学检测。
DOI：
10.1177/31.3.6186733
发表时间：
1983
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Schulte,BA;Spicer,SS
通讯作者：
Spicer,SS