Competitive adsorption of proteins and low-molecular-weight surfactants: computer simulation and microscopic imaging.
Competitive adsorption of proteins and low-molecular-weight surfactants: computer simulation and microscopic imaging.
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DOI：
10.1016/j.cis.2003.08.003
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发表时间：
2003-10
影响因子：
15.6
通讯作者：
L. Pugnaloni;E. Dickinson;R. Ettelaie;A. Mackie;P. Wilde
中科院分区：
文献类型：
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作者：
L. Pugnaloni;E. Dickinson;R. Ettelaie;A. Mackie;P. Wilde
蛋白质和低分子(LMW)表面活性剂在食品工业中用作乳化(和起泡)成分和稳定剂。这些属性与它们在流体-流体(和气液)界面上的吸附能力有关，降低了液体的界面(和表面)张力。因此，对这些分子的吸附层性质的研究有望有助于更好地了解它们对食品的影响。直接证明蛋白质和低分子表面活性剂体系介观模型的有效性，只能通过与宏观和介观实验相对照的定量理论预测来实现。计算机模拟是从数学上预测现实复杂性模型行为的为数不多的可用工具之一。此外，原子力显微镜(AFM)等实验技术现在可以对这些系统进行高分辨率成像，提供与模拟相比较的介观尺度测量。在这篇综述中，我们汇集了过去几年在这个介观层面上产生的一些相关发现。描述了一个由球形粒子通过键合和非键合作用力相互作用而成的简单模型，并将计算机模拟结果与成像实验结果进行了比较。特别关注蛋白质二元混合物、低分子表面活性剂混合物，以及蛋白质+表面活性剂混合体系的吸附。我们认为，有必要进一步发展这些数学上定义明确的物理模型，以便对所涉及的关键物理化学过程有一个适当的了解。
Proteins and low-molecular-weight (LMW) surfactants are used in the food industry as emulsifying (and foaming) ingredients and as stabilizers. These attributes are related to their ability to adsorb at fluid–fluid (and gas–fluid) interfaces lowering the interfacial (and surface) tension of liquids. Hence, the study of the properties of adsorbed layers of these molecules can be expected to lead to a better understanding of their effect on food products. Direct proof of the validity of mesoscopic models of systems of proteins and LMW surfactants can only be achieved by quantitative theoretical predictions being tested against both macroscopic and mesoscopic experiments. Computer simulation constitutes one of the few available tools to predict mathematically the behaviour of models of realistic complexity. Furthermore, experimental techniques such as atomic force microscopy (AFM) now allow high resolution imaging of these systems, providing the mesoscopic scale measurements to compare with the simulations. In this review, we bring together a number of related findings that have been generated at this mesoscopic level over the past few years. A useful simple model consisting of spherical particles interacting via bonded and unbonded forces is described, and the derived computer simulation results are compared against those from the imaging experiments. Special attention is paid to the adsorption of binary mixtures of proteins, mixtures of LMW surfactants, and also protein+surfactant mixed systems. We believe that further development of these mathematically well-defined physical models is necessary in order to achieve a proper understanding of the key physico–chemical processes involved.