Arbuscular mycorrhizal fungal communities are phylogenetically clustered at small scales
Arbuscular mycorrhizal fungal communities are phylogenetically clustered at small scales
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DOI：
10.1038/ismej.2014.72
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发表时间：
2014-11-01
期刊：
影响因子：
11
通讯作者：
Hempel, Stefan
中科院分区：
文献类型：
--
作者：
Horn, Sebastian;Caruso, Tancredi;Hempel, Stefan
利用覆盖球囊霉门全基因组的新一代测序技术，研究了与短毛羊茅（Festuca brevipila）相关的丛枝菌根真菌（AMF）的系统发育群落结构。研究系统是一个半干旱草原，具有较高的植物多样性和陡峭的环境梯度的pH值，C，N，P和土壤水分含量。分别分析了根和根际土壤中AMF的群落结构，共包括74个不同的操作分类单位（OTU）。社区水平的方差划分表明，在多尺度的空间自相关控制时，环境因素在确定AM物种组成的作用是边际。相反，系统发育距离和空间距离是AMF群落的主要相关因素：更密切相关（因此可能具有相似性状）的OTU更有可能共同出现。这种模式是不敏感的系统发育抽样宽度。考虑到环境的轻微影响，我们建议在小尺度上密切相关的AMF正相关，通过生物因素，如植物AMF过滤和土壤生物群内的相互作用。
Next-generation sequencing technologies with markers covering the full Glomeromycota phylum were used to uncover phylogenetic community structure of arbuscular mycorrhizal fungi (AMF) associated with Festuca brevipila. The study system was a semi-arid grassland with high plant diversity and a steep environmental gradient in pH, C, N, P and soil water content. The AMF community in roots and rhizosphere soil were analyzed separately and consisted of 74 distinct operational taxonomic units (OTUs) in total. Community-level variance partitioning showed that the role of environmental factors in determining AM species composition was marginal when controlling for spatial autocorrelation at multiple scales. Instead, phylogenetic distance and spatial distance were major correlates of AMF communities: OTUs that were more closely related (and which therefore may have similar traits) were more likely to co-occur. This pattern was insensitive to phylogenetic sampling breadth. Given the minor effects of the environment, we propose that at small scales closely related AMF positively associate through biotic factors such as plant-AMF filtering and interactions within the soil biota.