Swietenia macrophylla extract promotes the ability of Caenorhabditis elegans to survive Pseudomonas aeruginosa infection
Swietenia macrophylla extract promotes the ability of Caenorhabditis elegans to survive Pseudomonas aeruginosa infection
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DOI：
10.1016/j.jep.2011.12.016
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发表时间：
2012-01-31
影响因子：
5.4
通讯作者：
Shu-Chien, Alexander Chong
中科院分区：
文献类型：
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作者：
Dharmalingam, Komalavali;Tan, Boon-Khai;Shu-Chien, Alexander Chong
民族药理学相关性：大叶桃花心木或俗称大叶桃花心木，传统上被用作抗菌和抗真菌剂。 研究目的：许多细菌物种中出现的抗生素耐药性问题表明需要发现新的抗感染药物。在这里，我们通过一项涉及机会性人类病原体铜绿假单胞菌致死感染秀丽隐杆线虫的试验研究了大叶桃花心木的抗感染特性。材料和方法：使用慢速杀灭试验，用感染性铜绿假单胞菌菌株 (PA14) 攻击秀丽隐杆线虫。通过比较提取物处理和未处理的蠕虫种群之间的存活百分比，评估了桃花心木种子乙酸乙酯提取物促进受感染蠕虫存活的能力。还评估了桃花心木对 PA14 生长、秀丽隐杆线虫摄食率和 PA14 在蠕虫肠道中定植程度的影响。最后，使用荧光转基因线虫菌株和实时 PCR，研究了桃花心木对免疫反应基因 lys-7 表达的影响。 结果：我们的结果证明了桃花心木种子乙酸乙酯提取物能够拯救线虫免受致命的 PA14 感染。因此，我们表明该提取物可促进存活，而不会表现出任何杀菌作用或干扰秀丽隐杆线虫的摄食率。我们还发现，桃花心木提取物能够恢复 PA14 感染的秀丽隐杆线虫中最初被抑制的 lys-7 水平。结论：桃花心木提取物能够增强秀丽隐杆线虫在 PA14 感染中的生存能力，而不直接杀死病原体。我们进一步表明，该提取物增强了秀丽隐杆线虫先天免疫关键基因的表达。总的来说，这些发现强烈表明桃花心木种子中存在能够降低铜绿假单胞菌毒力和/或增强宿主抵抗力的化合物。 (C) 2011 Elsevier Ireland Ltd. 保留所有权利。
Ethnopharmacological relevance: Swietenia macrophylla or commonly known as big leaf mahogany, has been traditionally used as an antibacterial and antifungal agent.Aim of the study: The unwanted problem of antibiotic resistance in many bacterial species advocates the need for the discovery of the new anti-infective drugs. Here, we investigated the anti-infective properties of Swietenia macrophylla with an assay involving lethal infection of Caenorhabditis elegans with the opportunistic human pathogen Pseudomonas aeruginosa.Materials and methods: Using a slow killing assay, Caenorhabditis elegans was challenged with an infective strain of Pseudomonas aeruginosa (PA14). The ability of Swietenia macrophylla seed ethyl acetate extract to promote the survival of infected worms was assessed by comparing the percentage of survival between extract treated and non-treated worm populations. The effect of Swietenia macrophylla towards PA14 growth, Caenorhabditis elegans feeding rate and degree of PA14 colonization in the worm gut was also evaluated. Lastly, using a fluorescent transgenic Caenorhabditis elegans strain and real time PCR, the effect of Swietenia macrophylla on the expression of lys-7, an immune response gene was also investigated.Results: Our results demonstrate the ability of Swietenia macrophylla seed ethyl acetate extract in rescuing Caenorhabditis elegans from fatal PA14 infection. Consequently, we showed that the extract promotes the survival without exhibiting any bactericidal effect or perturbation of Caenorhabditis elegans feeding rate. We also showed that Swietenia macrophylla was able to restore the initially repressed lys-7 level in PA14 infected Caenorhabditis elegans.Conclusion: Swietenia macrophylla extract is able to enhance the ability of Caenorhabditis elegans to survive PA14 infection without directly killing the pathogen. We further showed that the extract boosted the expression of a gene pivotal for innate immunity in Caenorhabditis elegans. Collectively, these findings strongly suggest the presence of compounds within Swietenia macrophylla seed that either reduces Pseudomonas aeruginosa virulence and/or enhance host resistance. (C) 2011 Elsevier Ireland Ltd. All rights reserved.