Human neutrophil gelatinase is a collagenase type IV.
Human neutrophil gelatinase is a collagenase type IV.
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人中性粒细胞明胶酶是 IV 型胶原酶。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
P. Vignais
中科院分区：
文献类型：
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作者：
F. Morel;S. Berthier;M. Guillot;P. Zaoui;C. Massoubre;F. Didier;P. Vignais
从人中性粒细胞分泌的明胶酶被纯化为94 kDa多肽。测定并比较了纯化蛋白的明胶溶解活性和IV型胶原溶解活性。从抗明胶酶的抗血清中纯化的免疫球蛋白抑制明胶酶和IV型胶原酶的活性。与Ⅰ型胶原酶的抑制作用无交叉反应，而重组金属蛋白酶组织抑制剂对三种金属蛋白酶的抑制作用相似。纯化的明胶酶降解变性的I型和天然IV型胶原蛋白;没有天然I型胶原蛋白的蛋白水解。
Secreted gelatinase from human neutrophils was purified as a 94 kDa polypeptide. Gelatinolytic and type IV collagenolytic activities of the purified protein were measured and compared. Immunoglobulins purified from antisera raised against gelatinase inhibited both the gelatinase and type IV collagenase activities. There was no cross-reaction in the inhibition with type I collagenase while the three metalloproteases were similarly inhibited by recombinant tissue inhibitor of metalloproteases. Purified gelatinase degraded denatured type I and native type IV collagens; there was no proteolysis of native type I collagen.