Augmentation of fracture healing by hydroxyapatite/collagen paste and bone morphogenetic protein-2 evaluated using a rat femur osteotomy model
Augmentation of fracture healing by hydroxyapatite/collagen paste and bone morphogenetic protein-2 evaluated using a rat femur osteotomy model
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DOI：
10.1002/jor.23646
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发表时间：
2018-01-01
影响因子：
2.8
通讯作者：
Sotome, Shinichi
中科院分区：
文献类型：
--
作者：
Wei, Xuetao;Egawa, Satoru;Sotome, Shinichi
在骨折治疗中，生物骨愈合通常取决于骨的自然骨折愈合能力，即使在手术治疗的病例中也是如此。羟基磷灰石/胶原复合材料（HAp/Col）具有高骨传导性和刺激成骨祖细胞。此外，它还具有吸附骨形态发生蛋白（BMPs）的能力。在本研究中，我们制备了一种可注射HAp/Col膏剂，并评估了其对骨愈合的增强作用。此外，还对HAp/Col膏体与BMP-2联合的效果进行了评价。我们采用大鼠股骨截骨模型，缺损尺寸为1mm。雄性Wistar大鼠分为以下四组：对照组、HAp/Col组、可吸收胶原海绵（ACS）植入BMP-2 （1g）组和HAp/Col植入BMP-2组。显微ct分析、三点弯曲试验和组织学评价。HAp/Col组8例中有2例实现骨愈合，ACS+BMP-2组8例中有5例实现骨愈合，HAp/Col+BMP-2组8例均在术后8周实现骨愈合。对照组未实现骨愈合。此外，在HAp/Col+BMP-2组中，融合股骨的生物力学强度与对侧完整股骨相当；截骨侧断裂点与对侧断裂点的机械载荷之比为1.00 +/- 0.151 （SD）。这些结果表明，HAp/Col膏体添加或不添加BMP-2可增强骨愈合。(c) 2017年骨科研究会。Wiley期刊公司出版。[J]中华骨科杂志，2018。
In fracture treatment, biological bone union generally depends on the bone's natural fracture healing capacity, even in surgically treated cases. Hydroxyapatite/collagen composite (HAp/Col) has high osteoconductivity and stimulates osteogenic progenitors. Furthermore, it has the potent capacity to adsorb bone morphogenetic proteins (BMPs). In this study, we prepared an injectable HAp/Col paste and evaluated its augmentation of bone union. Furthermore, the effect of HAp/Col paste combined with BMP-2 was also evaluated. We used a rat femur osteotomy model with a defect size of 1mm. Male Wistar rats were assigned to one of the following four groups; a control group without any implant, a HAp/Col implant group, a group that received an absorbable collagen sponge (ACS) implant impregnated with BMP-2 (1g), and a group that received a HAp/Col implant impregnated with BMP-2 implant. Micro-CT analysis, three-point bending tests, and histological evaluation were performed. Bone union was achieved in two of eight cases in the HAp/Col group, five of eight cases in the ACS+BMP-2 group, and all cases in the HAp/Col+BMP-2 group at 8 weeks post-surgery. The control group did not achieve bone union. In addition, in the HAp/Col+BMP-2 group, the biomechanical strength of the fused femurs was comparable to that of the contralateral intact femur; the ratio of the mechanical load at the breaking point of the osteotomy side relative to that of the contralateral side was 1.00 +/- 0.151 (SD). These results indicate that HAp/Col paste with or without BMP-2 augments bone union. (c) 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:129-137, 2018.