RASSF1A gene promoter methylation in esophageal cancer specimens
RASSF1A gene promoter methylation in esophageal cancer specimens
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DOI：
10.1111/j.1442-2050.2005.00501.x
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发表时间：
2005-01-01
影响因子：
2.6
通讯作者：
Kuwano, H
中科院分区：
文献类型：
--
作者：
Yamaguchi, S;Kato, H;Kuwano, H
RASSF1A在人类癌症中经常因启动子甲基化而失活。为了了解RASSF1A基因在食管鳞状细胞癌（ESCC）中的作用，我们研究了原发性ESCC中RASSF1A基因的甲基化，以确定这种表观遗传畸变的频率及其临床病理学意义。应用甲基化特异性聚合酶链反应（MSP）检测55例食管鳞癌组织中RASSF1A基因甲基化状态。RASSF1A基因甲基化在55例原发性ESCC中发现13例（24%）。原发性食管鳞癌RASSF1A基因启动子区甲基化与年龄、性别、肿瘤位置、浸润深度和肿瘤分期无关。发现与肿瘤分化相关。与预后无相关性。总之，这表明RASSF1A基因的失活，由于启动子甲基化与食管鳞癌的肿瘤去分化。
RASSF1A is frequently inactivated by promoter methylation in human cancers. To understand the involvement of the RASSF1A gene in esophageal squamous cell cancer (ESCC), we investigated the methylation of the RASSF1A gene in primary ESCC to define the frequency of this epigenetic aberration and its clinicopathological significance. Methylation-specific polymerase chain reaction (MSP) was used to detect RASSF1A gene methylation in DNA from 55 cases of ESCC. Methylation of the RASSF1A gene was found in 13 of 55 (24%) cases of primary ESCC. No association was found between the promoter methylation of the RASSF1A gene in primary ESCC and age, gender, localization, invasion depth, or tumor stage. Association was found with tumor differentiation. There was no correlation with its prognosis. In conclusion, it was suggested that an inactivation of the RASSF1A gene due to promoter methylation was associated with de-differentiation of the tumor in ESCC.