FcγRIIB-nt645+25A? G gene polymorphism and periodontitis in Japanese women with preeclampsia
FcγRIIB-nt645+25A? G gene polymorphism and periodontitis in Japanese women with preeclampsia
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FcγRIIB-nt645+25A?G基因多态性与日本子痫前期女性牙周炎
DOI：
10.1111/j.1744-313x.2012.01124.x
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发表时间：
2012
影响因子：
2.2
通讯作者：
他5名
中科院分区：
文献类型：
--
作者：
Wang Y;Sugita N(2番目);Kikuchi A(3番目);Yoshie H(9番目);他5名
FcγRIIB含有独特的免疫受体酪氨酸基抑制基序（ITIM），作为白细胞活化和抗体产生的负反馈调节剂发挥作用。我们以前曾报道FcγRIIB-nt 645 + 25 A/G基因多态性与牙周炎的患病率和严重程度、外周血B淋巴细胞上FcγRIIB表达水平和血清抗牙周致病菌IgG水平相关。以前的研究报告说，产妇牙周病与先兆子痫的风险增加有关。因此，FcγRIIB‐ nt 645 + 25 A/G基因多态性可能通过影响孕妇对牙周致病菌的免疫应答而与子痫前期相关。为了阐明FcγRIIB‐ nt 645 + 25 A/G基因多态性是否与日本孕妇的先兆子痫和/或牙周炎相关，对先兆子痫（n= 13）和无先兆子痫（n= 106）的女性进行了病例对照研究。 在分娩后5天内收集孕妇牙周参数和龈下菌斑中伴放线菌聚集杆菌、牙龈卟啉单胞菌和中间普氏菌的细菌数据。 采用外周血基因组DNA检测FcγR基因型。测定每种细菌特异性的血清IgG水平。FcγRIIB‐ nt 645 + 25 A/G多态性与子痫前期的发生有显著相关性（P= 0.013）。 先兆子痫组FcγRIIB‐ nt 645 + 25 AA基因型频率高于非先兆子痫组（P= 0.007）。 先兆子痫组龈下菌斑中伴放线菌DNA含量高于对照组（P=0.017）。总之，母体FcγRIIB-nt 645 + 25 A/G多态性和龈下伴放线放线菌DNA水平与少数独立于牙周感染的日本女性中先兆子痫的患病率显著相关。应进行进一步的调查，以确认在更大的人群中的这种关联，并确定该关联的生物学过程。
FcγRIIB contains a unique immunoreceptor tyrosine‐based inhibition motif (ITIM) and functions as a negative feedback regulator of leucocyte activation and antibody production. We have previously reported FcγRIIB‐nt645+25A/G gene polymorphism to be associated with prevalence and severity of periodontitis, FcγRIIB expression level on peripheral B lymphocytes and the serum IgG level against periodontopathic bacteria. Previous studies have reported maternal periodontal disease to be associated with an increased risk for preeclampsia. Therefore, FcγRIIB‐nt645+25A/G gene polymorphism may be associated with preeclampsia by affecting immune response to periodontopathic bacteria in pregnant women. To elucidate whether FcγRIIB‐nt645+25A/G gene polymorphism has associations with preeclampsia and/or periodontitis in pregnant Japanese women, a case–control study was carried out on women with preeclampsia (n= 13) and without preeclampsia (n= 106). Maternal periodontal parameters and bacterial data ofAggregatibacter actinomycetemcomitans, Porphyromonas gingivalisandPrevotella intermediain subgingival plaque were collected within 5 days of delivery. FcγR genotypes of each woman were determined using the genomic DNA isolated from peripheral blood. Serum IgG levels specific for each bacteria were determined. There was a significant association between FcγRIIB‐nt645+25A/G polymorphism and preeclampsia (P= 0.013). The frequency of the FcγRIIB‐nt645+25AA genotype was higher in the preeclampsia group compared with the nonpreeclampsia group (P= 0.007). The DNA level ofA. actinomycetemcomitansfrom subgingival plaque was shown to be higher in the preeclampsia group (P=0.017). In conclusion, maternal FcγRIIB‐nt645+25A/G polymorphism and subgingival DNA level ofA. actinomycetemcomitanswere significantly associated with the prevalence of preeclampsia in a limited number of Japanese women independently with periodontal infection. Further investigations should be performed to confirm this association in a larger population and to determine the biological process of the association.