Methodological considerations for measuring biofluid-based microRNA biomarkers.
Methodological considerations for measuring biofluid-based microRNA biomarkers.
复制标题
测量基于生物流体的microRNA生物标志物的方法学考虑。
DOI：
10.1080/10408444.2021.1907530
复制
发表时间：
2021-03
影响因子：
5.9
通讯作者：
Harrill AH
中科院分区：
文献类型：
--
作者：
Chorley BN;Atabakhsh E;Doran G;Gautier JC;Ellinger-Ziegelbauer H;Jackson D;Sharapova T;Yuen PST;Church RJ;Couttet P;Froetschl R;McDuffie J;Martinez V;Pande P;Peel L;Rafferty C;Simutis FJ;Harrill AH
MicroRNAs(MiRNAs)是一种小的非编码RNA，调节信使RNA的表达，参与几乎所有的细胞过程。重要的是，miRNAs可以在细胞外释放，并在这些基质中稳定存在，它们可以作为器官或细胞特异性毒性、疾病和生物状态的指示剂。因此，出于科学、调控和临床目的，人们对开发miRNAs作为不良后果的生物标志物的热情很高。尽管miRNAs的测量能力取得了进步，但miRNAs仍未被常规用作非侵入性生物标记物。这在一定程度上是由于缺乏样品制备和miRNA测量的标准方法，以及对它们的生物学解释的不确定性。卫生与环境科学研究所(HESI)新兴系统毒理学风险评估委员会(ESTAR)内的microRNA生物标记物工作组成员是一个由私营和公共部门的科学家组成的联盟，致力于开发miRNAs作为应用生物标记物。在这里，我们探讨常规接受和使用miRNA生物标记物的主要障碍，以及开发中成功和不足的案例。最后，我们提供了对miRNA测量、收集和分析工具的见解，为解决常规生物标记物使用方面的知识差距提供了坚实的基础。
MicroRNAs (miRNAs) are small non-coding RNA that regulate the expression of messenger RNA and are implicated in almost all cellular processes. Importantly, miRNAs are can be released extracellularly and are stable in these matrices where they may serve as indicators of organ or cell-specific toxicity, disease, and biological status. There has thus been great enthusiasm for developing miRNAs as biomarkers of adverse outcomes for scientific, regulatory, and clinical purposes. Despite advances in measurement capabilities for miRNAs, miRNAs are still not routinely employed as non-invasive biomarkers. This is in part due to the lack of standard approaches for sample preparation and miRNA measurement and uncertainty in their biological interpretation. Members of the microRNA Biomarkers Workgroup within the Health and Environmental Sciences Institute’s (HESI) Committee on Emerging Systems Toxicology for the Assessment of Risk (eSTAR) are a consortium of private- and public-sector scientists dedicated to developing miRNAs as applied biomarkers. Here, we explore major impediments to routine acceptance and use of miRNA biomarkers and case examples of success and deficiencies in development. Finally, we provide insight on miRNA measurement, collection, and analysis tools to provide solid footing for addressing knowledge gaps toward routine biomarker use.
登录
查看更多内容
影响因子：
13.5
作者：
Antoine, Daniel J.;Dear, James W.;Lewis, Philip Starkey;Platt, Vivien;Coyle, Judy;Masson, Moyra;Thanacoody, Ruben H.;Gray, Alasdair J.;Webb, David J.;Moggs, Jonathan G.;Bateman, D. Nicholas;Goldring, Christopher E.;Park, B. Kevin
通讯作者：
Park, B. Kevin
影响因子：
3.7
作者：
Meyer SU;Kaiser S;Wagner C;Thirion C;Pfaffl MW
通讯作者：
Pfaffl MW
影响因子：
2.2
作者：
Amur, Shashi G.;Sanyal, Sarmistha;Buckman-Garner, ShaAvhree Y.
通讯作者：
Buckman-Garner, ShaAvhree Y.
DOI：
10.1093/toxsci/kfaa181
发表时间：
2021-02-26
期刊：
Toxicological sciences : an official journal of the Society of Toxicology
影响因子：
--
作者：
Chorley BN;Ellinger-Ziegelbauer H;Tackett M;Simutis FJ;Harrill AH;McDuffie J;Atabakhsh E;Nassirpour R;Whiteley LO;Léonard JF;Carswell GK;Harpur E;Chen CL;Gautier JC
通讯作者：
Gautier JC
影响因子：
4.4
作者：
Bushel PR;Caiment F;Wu H;O'Lone R;Day F;Calley J;Smith A;Li J
通讯作者：
Li J