Comparative genomics of emerging human ehrlichiosis agents.
Comparative genomics of emerging human ehrlichiosis agents.
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DOI：
10.1371/journal.pgen.0020021
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发表时间：
2006-02
期刊：
影响因子：
4.5
通讯作者：
Tettelin H
中科院分区：
文献类型：
--
作者：
Dunning Hotopp JC;Lin M;Madupu R;Crabtree J;Angiuoli SV;Eisen JA;Seshadri R;Ren Q;Wu M;Utterback TR;Smith S;Lewis M;Khouri H;Zhang C;Niu H;Lin Q;Ohashi N;Zhi N;Nelson W;Brinkac LM;Dodson RJ;Rosovitz MJ;Sundaram J;Daugherty SC;Davidsen T;Durkin AS;Gwinn M;Haft DH;Selengut JD;Sullivan SA;Zafar N;Zhou L;Benahmed F;Forberger H;Halpin R;Mulligan S;Robinson J;White O;Rikihisa Y;Tettelin H
Anaplasma（以前为Ehrlichia）吞噬细胞，Ehrlichia chaffeensis和Neorickettsia（以前是Ehrlichia）Sennetsu是引起人类Ehrllichiosis的细胞内载体传播病原体，是一种新兴的感染性疾病。我们介绍了这些生物的完整基因组序列，以及与立克序列中其他生物体的比较。 Ehrlichia spp。和Anaplasma spp。显示出促进抗原变异的免疫主导外膜蛋白的独特大扩张。与最接近的自由生活亲戚相比，所有立克人士的合成氨基酸的能力都降低。与立克西西科家族的成员不同，这些致病性无质膜科能够使所有主要的维生素，辅助因子和核苷酸都能赋予无脊椎动物载体或脊椎动物宿主的有益作用。进一步的分析确定了潜在地参与液泡隔离的蛋白质，这是涉及毒性载体的生命周期，脊椎动物发病机理，人体发病机理和缺乏跨囊的传播。这些发现为这些强制性细胞内病原体的生物学提供了重要的见解。
ehrllichiosis是一种急性疾病，可触发人类和动物的流感样症状。它是由tick或Flukes传播的一系列细菌引起的。但是，由于这些细菌很难培养，因此对生物体的理解很少。测序了三种新出现的人类病原体的基因组。设计一个数据库允许将这三个基因组与具有相似生活方式的其他16种细菌进行比较。来自该数据库的分析揭示了新的物种特异性和疾病特异性基因，表明适应性适应性，致病性状和其他特征。特别是，其中一种生物包含与宿主相互作用的100多个单个基因的副本。这些比较还得以重建这些细菌及其近亲的五个代表性基因组的代谢潜力。通过这项工作，科学家可以认真研究这些新兴病原体。
Anaplasma (formerly Ehrlichia) phagocytophilum, Ehrlichia chaffeensis, and Neorickettsia (formerly Ehrlichia) sennetsu are intracellular vector-borne pathogens that cause human ehrlichiosis, an emerging infectious disease. We present the complete genome sequences of these organisms along with comparisons to other organisms in the Rickettsiales order. Ehrlichia spp. and Anaplasma spp. display a unique large expansion of immunodominant outer membrane proteins facilitating antigenic variation. All Rickettsiales have a diminished ability to synthesize amino acids compared to their closest free-living relatives. Unlike members of the Rickettsiaceae family, these pathogenic Anaplasmataceae are capable of making all major vitamins, cofactors, and nucleotides, which could confer a beneficial role in the invertebrate vector or the vertebrate host. Further analysis identified proteins potentially involved in vacuole confinement of the Anaplasmataceae, a life cycle involving a hematophagous vector, vertebrate pathogenesis, human pathogenesis, and lack of transovarial transmission. These discoveries provide significant insights into the biology of these obligate intracellular pathogens. Ehrlichiosis is an acute disease that triggers flu-like symptoms in both humans and animals. It is caused by a range of bacteria transmitted by ticks or flukes. Because these bacteria are difficult to culture, however, the organisms are poorly understood. The genomes of three emerging human pathogens causing ehrlichiosis were sequenced. A database was designed to allow the comparison of these three genomes to sixteen other bacteria with similar lifestyles. Analysis from this database reveals new species-specific and disease-specific genes indicating niche adaptations, pathogenic traits, and other features. In particular, one of the organisms contains more than 100 copies of a single gene involved in interactions with the host(s). These comparisons also enabled a reconstruction of the metabolic potential of five representative genomes from these bacteria and their close relatives. With this work, scientists can study these emerging pathogens in earnest.