HISTOCOMPATIBILITY‐2 SYSTEM IN WILD MICE: I. Identification of Five New H‐2 Chromosomes
HISTOCOMPATIBILITY‐2 SYSTEM IN WILD MICE: I. Identification of Five New H‐2 Chromosomes
复制标题
野生小鼠的组织相容性 2 系统：I. 5 条新 H-2 染色体的鉴定
作者：
J. Klein
将安娜堡地区养殖场捕获的5只野鼠反复回交到B10.BR品系，通过SS标记筛选野生H-2染色体，获得B10.W品系(即B10.GAA20、B10.KEA5、B10.KPA42、B10.KPB68、B10.SAA48)。用B10.W免疫C3H型和B10A型小鼠，制备抗5种野生型抗原的抗血清。这五种抗原的遗传决定因素被证明与SS基因座紧密连锁，因此，显然是H-2系统的一部分。在所有测试的26个自交系中，这些抗原都不存在。在野鼠中，它们的出现被重新归类到最初的野生父母所在的种群。结论是，这些抗原代表了H-2的新的私有特异性，符号H-2.101到H-2.105被指定给H-2。相应的H-2染色体命名为H-8wa至H-2We。用近交系衍生试剂进行的血清学分析表明，新的H-2染色体除了确定H-2.100系列的抗原外，还确定了来自近交系的已知抗原。然而，与近交系共有的抗原都是公共抗原类别的成员。在5个B10.W品系中未发现近交系来源的私有抗原。讨论了这些发现对解释公共抗原和私人抗原、野生鼠种群的繁殖结构和H-2多态的评估的意义。
Five wild mice, captured on farms in the Ann Arbor area, were repeatedly back-crossed to strain B10.BR, the wild-derived H-2 chromosomes were selected through the Ss marker, and B10.W (i.e., B10.GAA20, B10.KEA5, B10.KPA42, B10.KPB68, B10.SAA48) lines were produced. Mice of strains C3H or B10.A were immunized with B10.W tissues, and antisera against five wild-derived antigens were prepared. The genetic determinants of these five antigens were shown to be closely linked with the Ss locus and, thus, apparently to be a part of the H-2 system. The antigens were absent in all 26 inbred strains tested. In wild mice, their occurrence was restrieted to the population from which the original wild parents came. It was concluded that the antigens represent new private H-2 specificities to which symbols H-2.101 through H-2.105 were assigned. The corresponding H-2 chromosomes were designated H-8wa through H-2we. Serological analyses with inbred-derived reagents revealed that the new H-2 chromosomes determined, in addition to the antigens of the H-2.100 series, antigens known from inbred strains. However, the antigens shared with the inbred strains were all members of the category of public antigens. Inbred-derived private antigens have not been found in the five B10.W lines. The implications of these findings on the interpretation of the public versus private antigens, the breeding structure of the wild mouse populations, and the assessment of the H-2 polymorphism are discussed.