Small molecule-induced trinucleotide repeat contractions during <i>in vitro</i> DNA synthesis
Small molecule-induced trinucleotide repeat contractions during <i>in vitro</i> DNA synthesis
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<i>体外</i> DNA 合成过程中小分子诱导的三核苷酸重复收缩
DOI：
10.1039/d1cc00349f
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发表时间：
2021
影响因子：
4.9
通讯作者：
Nakatani Kazuhiko
中科院分区：
文献类型：
--
作者：
Dohno Chikara;Hagihara Masaki;Binti Mohd Zaifuddin Nursakinah;Nihei Mizuki;Saito Kaoru;Nakatani Kazuhiko
我们证明了选择性结合5′-CAG-3′/5′-CAG-3′三联体的合成配体NA在DNA聚合酶介导的引物延伸通过CAG重复模板期间诱导重复收缩。毛细管电泳和测序分析表明，在NA存在下，d（CAG）20模板产生主要含有3-6个CTG单元的缩短的新生链。
We demonstrated that a synthetic ligand NA, which selectively binds to a 5′-CAG-3′/5′-CAG-3′ triad, induced repeat contractions during DNA polymerase-mediated primer extension through the CAG repeat template. A thorough capillary electrophoresis and sequencing analysis revealed that the d(CAG)20 template gave shortened nascent strands mainly containing 3–6 CTG units in the presence of NA.